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Shelf Life of Trichoderma Mutant Inoculum with Dried Mud and Glutinous Rice as Carrier Materials
Abstract
Drying to produce powdered inoculum can damage Trichoderma spores and reduce spore viability, a factor commonly affected by carrier materials. This study investigated the storage capacity of Trichoderma mutant inoculum with dried mud and glutinous rice as carrier materials. Dried Trichoderma AA1 mutant spores were mixed with the carrier in the form of a mixture of dried mud and glutinous rice flour. There were 6 ratios of dried mud: glutinous rice, including 0:10, 2:8, 4:6, 6:4, 8:2, and 10:0. The spore-carrier mixtures were stored and analyzed for an additional 16 weeks. The availability of inoculum in all combinations of treatment was above 3.19×107 CFU (Colony Forming Units). The smallest availability fluctuations were observed at a ratio of 4:6, ranging from 3.54×107 to 6.1×107 CFU. Cellulase enzyme activity increased by 66.67% during 16 weeks of storage. The research suggests that Trichoderma with a ratio of dried mud: glutinous rice flour of 4:6 had an availability of 3.54×107 CFU and 0.020 ?mol/minute glucose-released carboxymethyl cellulase (CMCase) activity after 16 weeks of storage.
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