Shelf Life of Trichoderma Mutant Inoculum with Dried Mud and Glutinous Rice as Carrier Materials

Ali Mursyid Wahyu Mulyono; Muhammad Husein; Adi Ratriyanto; Sri Sukaryani; Novian Wely Asmoro; Afriyanti Afriyanti

doi:10.14334/jitv.v30i3.3443

Shelf Life of Trichoderma Mutant Inoculum with Dried Mud and Glutinous Rice as Carrier Materials

Ali Mursyid Wahyu Mulyono, Muhammad Husein, Adi Ratriyanto, Sri Sukaryani, Novian Wely Asmoro, Afriyanti Afriyanti

DOI

https://doi.org/10.14334/jitv.v30i3.3443

Abstract

Making powdered inoculum through the drying process will cause cell damage to Trichoderma spores and reducing spore viability, it is affected by the carrier material. This study investigated the storage capacity of Trichoderma mutant inoculum with dried mud and glutinous rice as carrier materials. Dried Trichoderma AA1 mutant spores were mixed with the carrier in the form of a mixture of dried mud and glutinous rice flour. There were 6 ratios of dried mud: glutinous rice, namely: 0:10, 2:8, 4:6, 6:4, 8:2, and 10:0. The mixtures of spores and carrier materials were stored and being analyzed until 16 weeks. The availability of inoculum in all combinations of treatment was above 3.19×107 CFU. The smallest availability fluctuations were shown at a ratio of 4:6 which ranged between 3.54×107 and 6.1×107 CFU. Cellulase enzyme activity increased during 16 weeks of storage with an increase of 66.67%. The research suggests Trichoderma with a ratio of dried mud: glutinous rice flour of 4:6 had an availability 3.54×107 CFU and 0.020 ?mol/minute glucose released carboxymethyl cellulase (CMCase) activity after 16 weeks storage.

References

Abuhena, Md, Md Golam Kabir, Md Faisal Azim, Jubair Al-Rashid, Noorain Munim Rasul, and Md Amdadul Huq. 2022. “A Stressing Method for Producing High-Density Trichoderma Spores in a Dual-Layer by Utilizing a Starch-Based Medium in a Reconditioning Approach.” Bioresource Technology Reports 19: 101165.

Adebami, Gboyega E, and Bukola C Adebayo-tayo. 2020. Development of Cellulolytic Strain by Genetic Engineering Approach for Enhanced Cellulase Production. Genetic and Metabolic Engineering for Improved Biofuel Production from Lignocellulosic Biomass. Elsevier Inc. https://doi.org/10.1016/B978-0-12-817953-6.00008-7.

Dewiyanti, Irma, Darmawi Darmawi, Zainal Abidin Muchlisin, Teuku Zahrial Helmi, and Iko Imelda Arisa. 2021. “Cellulase Activity of Bacteria Isolated from Water of Mangrove Ecosystem in Aceh Province.” Jurnal Ilmu-Ilmu Perikanan 10 (December): 243–50. https://doi.org/10.13170/depik.10.3.22964.

Dimitrova, Katya Petkova. 2020. “Philosophy at The Use of Exogenous Xylanase and Dietary Fibre for Modern Broiler Chicken Production.” Harper Adams University.

Gooruee, Ramin, Mohammad Hojjati, Behrooz Alizadeh Behbahani, Samira Shahbazi, and Hamed Askari. 2024. “Extracellular Enzyme Production by Different Species of Trichoderma Fungus for Lemon Peel Waste Bioconversion.” Biomass Conversion and Biorefinery 14 (2): 2777–86. https://doi.org/10.1007/s13399-022-02626-7.

Gruji?, Marica, Biljana Dojnov, Ivana Poto?nik, Lea Atanasova, Bojan Duduk, and Ewald Srebotnik. 2019. “Superior Cellulolytic Activity of Trichoderma Guizhouense on Raw Wheat Straw.” World Journal of Microbiology and Biotechnology. https://doi.org/10.1007/s11274-019-2774-y.

Iannaccone, Francesco, Vittoria Alborino, Irene Dini, Anna Balestrieri, Roberta Marra, Rosario Davino, Antonio Di Francia, Felicia Masucci, Francesco Serrapica, and Francesco Vinale. 2022. “In Vitro Application of Exogenous Fibrolytic Enzymes from Trichoderma Spp . to Improve Feed Utilization by Ruminants,” 1–16.

Iqbal, Shomaila, Muhammad Ashfaq, Aamir H Malik, Muhammad Inam, and Khalid S Khan. 2022. “MORPHO-MOLECULAR CHARACTERIZATION OF TRICHODERMA ISOLATES FROM RHIZOSPHERIC SOILS OF VEGETABLES IN PAKISTAN.” International Journal of Phytopathology 11 (03): 253–66. https://doi.org/10.33687/phytopath.011.03.4309.

Iqbal, Shomaila, Muhammad Ashfaq, and Aamir Humayun Malik. 2017. “Isolation , Preservation and Revival of Trichoderma Viride in Culture Media” 5 (3): 1640–46.

Jayasekara, Sandhya, and Renuka Ratnayake. 2019. “Microbial Cellulases: An Overview and Applications.” Cellulose 22 (92): 10–5772.

Jha, Rajesh. 2021. “Dietary Fiber in Poultry Nutrition and Their Effects on Nutrient Utilization , Performance , Gut Health , and on the Environment : A Review” 0: 1–16.

Kaushal, Sonia, and Sunita Chandel. 2017. “Enhancing the Shelf Life of Trichoderma Species by Adding Antioxidants Producing Crops to Various Substrates” 6 (3): 307–14.

Khan, Mujeebur R, and Fayaz A Mohiddin. 2018. Trichoderma : Its Multifarious Utility in Crop Improvement. Crop Improvement through Microbial Biotechnology. Elsevier B.V. https://doi.org/10.1016/B978-0-444-63987-5.00013-X.

Martinez, Yolanda, Javier Ribera, Francis W M R Schwarze, and Kevin De France. 2023. “Biotechnological Development of Trichoderma ? Based Formulations for Biological Control.” Applied Microbiology and Biotechnology 107 (18): 5595–5612. https://doi.org/10.1007/s00253-023-12687-x.

Miller, Gail Lorenz. 1959. “Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar.” Analytical Chemistry 31 (3): 426–28. https://doi.org/10.1021/ac60147a030.

Mulyono, Ali Mursyid Wahyu. 2008. “MUTAN JAMUR SELULOLITIK Trichoderma Sp. UNTUK MENINGKATKAN KUALITAS ONGGOK SEBAGAI BAHAN PAKAN AYAM BROILER,” 1–161.

Munir, Shahzad, Qaiser Jamal, Kalsoom Bano, and Sikandar Khan Sherwani. 2013. “Biocontrol Ability of Trichoderma.” Iternational Journal of Agriculture and Crop Sciences 6 (18): 1246–52.

Oeng, Sopheap, Jean-bafptiste Ronat, Jeanne Orekan, Barbara Barb, Joanne Letchford, Jan Jacobs, Dissou Affolabi, and Liselotte Hardy. 2021. “Culture Media for Clinical Bacteriology in Low- and Middle-Income Countries : Challenges , Best Practices for Preparation and Recommendations for Improved Access” 27: 1400–1408. https://doi.org/10.1016/j.cmi.2021.05.016.

Pandya, Bhavika, and Susy Albert. 2014. “Evaluation of Trichoderma Reesei as a Compatible Partner with Some White Rot Fungi for Potential Bio-Bleaching in Paper Industry.” Annals of Biological Research 5 (4): 43–51.

Patagundi, Basavaraj I, C T Shivasharan, and B B Kaliwal. 2014. “Isolation and Characterization of Cellulase Producing Bacteria from Soil.” Int.J.Curr.Microbiol.App.Sci 3 (5): 59–69.

Rajput, Abdul Qayoom, Muhammad Ali Khanzada, and Saleem Shahzad. 2014. “Effect of Different Substrates and Carbon and Nitrogen Sources on Growth and Shelf Life of Trichoderma Pseudokoningii.” INTERNATIONAL JOURNAL OF AGRICULTURE & BIOLOGY 16 (5): 893-899file:///C:/Users/lenovo/Downloads/S03014797.

Rimkus, Alina, Agne Namina, Marija Tereze Dzierkale, Oskars Grigs, Maris Senkovs, and Simona Larsson. 2023. “Impact of Growth Conditions on the Viability of Trichoderma Asperellum during Storage,” 1–11.

Rodrigo, Allyson, De Oliveira Lopes, Gabriel Olivo Locatelli, Raquel De Melo, Murillo Lobo Junior, Gabriel Moura Mascarin, and Christine Lamenha. 2020. “Preparation, Characterization and Cell Viability of Encapsulated Trichoderma Asperellum in Alginate Beads” 2048. https://doi.org/10.1080/02652048.2020.1729884.

Sarhan, A.R.T. 2015. “Biomass Production of Fungal and Bacterial Bio-Control Agents Using Various Agro Wastes as Natural Culture Media.” Egyptian Journal of Biological Pest Control 25 (2): 457–62.

Singh, Garima, Abhay Tiwari, Akansha Gupta, Ashwani Kumar, and P Hariprasad. 2021. “Bioformulation Development via Valorizing Silica-Rich Spent Mushroom Substrate with Trichoderma Asperellum for Plant Nutrient and Disease Management.” Journal of Environmental Management 297 (February): 113278. https://doi.org/10.1016/j.jenvman.2021.113278.

Singh, Raj, Anju Rani, Permod Kumar, Gyanika Shukla, and Amit Kumar. 2017. “CELLULOLYTIC ACTIVITY IN MICROORGANISMS” 36 (1). https://doi.org/10.5958/2320-3196.2017.00004.0.

Singh, Ramji, Ajay Tomer, Durga Prasad, and H S Viswanath. 2020. “Biodiversity of Trichoderma Species in Different Agro-Ecological Habitats.” Trichoderma: Agricultural Applications and Beyond, 21–40.

Siti, Sharifah, Maryam Syd, Abdul Rahman, Nur Ain, Izzati Mohd, Asma Aris, Nor Azwady, Abd Aziz, and Cameron Highland. 2021. “Malaysian Journal of Microbiology Morphological and Molecular Characterization of Trichoderma Species Isolated from Rhizosphere Soils in Malaysia” 17 (1): 80–89.

Steel, R. G. D., J. H. Torrie, and D. A. Dickey. 1996. Principles and Procedures of Statistics: A Biometrical Approach. New York: McGraw-Hill.

Strakowska, Judyta, Lidia B?aszczyk, and Jerzy Che?kowski. 2014. “The Significance of Cellulolytic Enzymes Produced by Trichoderma in Opportunistic Lifestyle of This Fungus.” Journal of Basic Microbiology 54 (SUPPL.1): 1–12. https://doi.org/10.1002/jobm.201300821.

Taye, Diriba, and Monenus Etefa. 2020. “Review on Improving Nutritive Value of Forage by Applying Exogenous Enzymes” 5 (6): 72–79.

Vimal, Joseph, Akhil Venu, and Jini Joseph. 2017. “Isolation and Identification of Cellulose Degrading Bacteria and Optimization of the Cellulase Production.” International Journal of Research in Biosciences 5 (3).

Zayed, M S, M Szumacher-Strabel, D A A El-Fattah, M A Madkour, M Gogulski, V Strompfová, A Cie?lak, and N E El-Bordeny. 2020. “Evaluation of Cellulolytic Exogenous Enzyme-Containing Microbial Inoculants as Feed Additives for Ruminant Rations Composed of Low-Quality Roughage.” Journal of Agricultural Science 158 (4). https://doi.org/10.1017/S0021859620000611.

Zope, V P, H P Jadhav, and R Z Sayyed. 2019. “Neem Cake Carrier Prolongs Shelf Life of Biocontrol Fungus Trichoderma Viridae” 57 (May): 372–75.