Diversity of  Myostatin Gene SNPc.267G>A and SNPc.111G>C as Candidate Genetic Markers for Growth Traits in Ongole Grade Cattle

Shidqi Muhammad Naufal; Ronny Rachman Noor; Jakaria Jakaria

doi:10.14334/jitv.v30i1.3417

Diversity of Myostatin Gene SNPc.267G>A and SNPc.111G>C as Candidate Genetic Markers for Growth Traits in Ongole Grade Cattle

Shidqi Muhammad Naufal, Ronny Rachman Noor, Jakaria Jakaria

DOI

https://doi.org/10.14334/jitv.v30i1.3417

Abstract

This study aims to analyze the diversity of the MSTN gene and its association with birth weight, weaning weight, and growth rate in PO cattle. A total of 77 blood samples from PO cattle, including 38 cows and 39 bulls, were obtained from the Balai Perbibitan dan Pengembangan Inseminasi Buatan Ternak Sapi Perah (BPPIB-TSP) in Ciamis. The diversity of SNP c.267G>A and c.111G>C was analyzed using the PCR-RFLP technique and the restriction enzymes HaeIII and AluI. Genotype frequencies, allele frequencies, heterozygosity values, and Hardy-Weinberg equilibrium were computed using the PopGen32 program. The t-test method assessed the association of SNP c.267G>A and c.111G>C with body weight. The results indicated genotype frequencies of AA, AG, and GG at 0.38, 0.56, and 0.06, respectively, for SNPc.267G>A, with allele frequencies of A and G at 0.66 and 0.34. For SNPc.111G>C, the genotype frequencies of CC, CG, and GG were 0.16, 0.29, and 0.56, respectively, while the allele frequencies for C and G were 0.30 and 0.70. Both SNPs were not in Hardy-Weinberg equilibrium. The SNPc.267G>A did not show an association with the body weight of male and female PO cattle. However, SNPc.111G>C was significantly associated with weaning weight and growth rate in male PO cattle. These findings suggest that the GG genotype of SNPc.111G>C may be a candidate genetic marker for selecting weaning weight and growth rate in PO bulls.

Keywords

Body Weight; Myostatin Gene; PO Cattle; SNP

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