Effects of Different Levels of Fish Meal in the Diet on Carcass Traits and Meat Quality of Bali Cattle

Paulus Klau Tahuk; Oktovianus R. Nahak; Gerson Frans Bira

doi:10.14334/jitv.v29i2.3431

Effects of Different Levels of Fish Meal in the Diet on Carcass Traits and Meat Quality of Bali Cattle

Paulus Klau Tahuk, Oktovianus R. Nahak, Gerson Frans Bira

DOI

https://doi.org/10.14334/jitv.v29i2.3431

Abstract

This study aimed to ascertain the impact of oocyte harvest methods and storage times on the amount, quality, and rate of buffalo oocyte maturation in vitro. Ovaries were collected from slaughterhouse at four different time storages (0, 3, 6, 9 and 12 h) before processing with three collection techniques slicing, aspiration, and slicing plus the aspiration of oocytes. According to the morphology of cumulus cell layers closely attached to the zona pellucida of the oocytes and the cytoplasmic appearance of the oocytes, the oocytes were divided into 4 groups (A, B, C, and D). Cumulus-oocyte-complexes (COCs) were transferred into in vitro maturation (IVM) medium of TCM- 199 (Sigma) for 24 h. The stages of nuclear maturation were examined by staining the enlarged COCs from each group. The result found that the average quantity of oocytes per ovary ranged from 8.08±1.28 to 9.83 ±1.89 and no significant effect (P>0.05) on the quantity of oocytes per ovary. The average of oocytes quality per ovary ranges from 0.2 to 5.00 was a highly significant effect of storage time on quality oocytes (P<0.01). The maturation rate in the M-II stage ranges from 1.33 to 3.92 per ovary, and storage time is a significant effect (P<0.05) on the maturation rate of local buffalo. The collection methods were highly significant (P<0.01) on the quantity of oocytes between slicing and aspiration, also between aspiration plus slicing, and no significant (P>0.05) between slicing and aspiration plus slicing. The Average oocyte quality ranged from 0.10 to 4.95 per ovary and the highest in slicing plus aspiration methods (P>0.05). The average maturation rate in M-II stage was ranged from 1.00 to 3.85 oocytes per ovary, and the methods of oocytes collection were significant (P<0.05) on the maturation rate of local buffalo. In conclusion that the storage time of up to 6 h and aspiration plus slicing is suitable on in vitro maturation of local buffalo.

Keywords

Bali Cattle; Carcass; Feed; Fish Meal; Meat Quality

Full Text:

PDF

References

Abdel-Khalek EA, El-Harairy MA, Shamiah ShM, Khalil WA. 2010. Effect of ovary preservation period on recovery rate and categories of dromedary camel oocytes. Saudi J Biol Sci. 17:231–235. DOI:10.1016/j.sjbs.2010.04.007.

Aguila L, Treulen F, Therrien J, Felmer R, Valdivia M, Smith LC. 2020. Oocyte selection for in vitro embryo production in Bovine species: Noninvasive approaches for new challenges of oocyte competence. Animals. 10:2196. DOI:10.3390/ani10122196.

Ahmed JA, Dutta D, Nashiruddullah N. 2015. Recovery of different Cumulus Oocyte Complex (COC) grades from Bovine ovaries by Aspiration method. J Anim Res. 5:631. DOI:10.5958/2277-940X.2015.00106.0.

Al-Shammari RFH, Almeeni IM. 2022. Effect of oocytes collection methods on oocytes quantity and quality in local Iraqi ewes. Int J Health Sci (Qassim). 6:14560–14567. DOI:10.53730/ijhs.v6nS1.10148.

Aquino LM, Manaois FV, Ocampo LC, Ocampo MB. 2015. Evaluation of bioassay using in vitro matured water buffalo oocytes in predicting bull sperm fertility. Journal of Agricultural Technology. 11:2531–2538.

Arul V. 2017. Effect of oocyte retrieval techniques on yield and quality of buffalo (Bubalus bubalus) oocytes. Int J Chem Stud. 5:1221–1225.

Barceló-Fimbres M, Campos-Chillón LF, Mtango NR, Altermatt J, Bonilla L, Koppang R, Verstegen JP. 2015. Improving in vitro maturation and pregnancy outcome in cattle using a novel oocyte shipping and maturation system not requiring a CO2 gas phase. Theriogenology. 84:109–117. DOI:10.1016/j.theriogenology.2015.02.020.

Chaudhari CF, Derashri HJ, Chaudhari SS, Puri G. 2014. Quantitative and qualitative study of buffalo oocytes recovered by different oocyte harvesting techniques. Indian J Anim Sci. 84. DOI:10.56093/ijans.v84i9.43522.

Deb GK, Dey SR, Nahar TN, Khan MYA, Rahman MM. 2016. In vitro production of bovine blastocyst with oocyte collected from abattoir ovary. Bangladesh J Anim Sci. 45:31–35. DOI:10.3329/bjas.v45i1.27485.

Ebrahimi B, Valojerdi MR, Eftekhari-Yazdi P, Baharvand H. 2010. In vitro maturation, apoptotic gene expression and incidence of numerical chromosomal abnormalities following cryotop vitrification of sheep cumulus-oocyte complexes. J Assist Reprod Genet. 27:239–246. DOI:10.1007/s10815-010-9401-z.

Elbaz HT, Abd-El-Razek EM, Genedy TM, Elweza AE, Zaghloul AH, Wagdy M. 2019. Effect of season and ovarian morphology of egyptian buffalo on oocyte quality and in vitro maturation rate. J Current Vet Res. 1:34–41.

Fitriani Z, Riyanto J, Dewanti R, Cahyadi M, Wati AK, Pawestri WP, Yanti Y. 2023. Reproductive performance of buffalo (Bubalus bubalis) in small scale farm in Boyolali Regency Central Java. JIIP. 33:327–335. DOI:10.21776/ub.jiip.2023.033.03.03.

Francesco DS, Boccia L, Di Palo R, Esposito G, Attanasio L, De Rosa A, Gasparrini B. 2007. Influence of temperature and time during ovary transportation on in vitro embryo production efficiency in the buffalo species (Bubalus bubalis). Ital J Anim Sci. 6:755–758. DOI:10.4081/ijas. 2007.s2.755.

Gabr ShA, Abdel-Khal AE, El-Ratel IT. 2014. Evaluation of some factors affecting quantity, quality and in vitro maturation of buffalo oocytes. Asian J Anim Vet Adv. 10:1–13. DOI:10.3923/ajava.2015.1.13.

Gad A, Abu Hamed S, Khalifa M, Amin A, El-Sayed A, Swiefy SA, El-Assal S. 2018. Retinoic acid improves maturation rate and upregulates the expression of antioxidant-related genes in in vitro matured buffalo (Bubalus bubalis) oocytes. Int J Vet Sci Med. 6:279–285. DOI10.1016 /j.ijvsm.2018.09.003.

Gilchrist G, Tscherner A, Nalpathamkalam T, Merico D, LaMarre J. 2016. MicroRNA Expression during Bovine Oocyte Maturation and Fertilization. Int J Mol Sci. 17(3):396. https://doi.org/10.3390/ijms17030396

Habeeb IA, Hussain SO. 2018. Comparative study between two media on in vitro maturation rate of local buffalo oocytes. Al-Qadisiyah J Vet Med Sci. 17:9–16. DOI:10.29079 /vol17iss1art466.

Hammad ME, Gabr SA, El-Ratel IT, Gad MA. 2014. Efficacy of different collection techniques on yield and quality of Egyptian buffalo oocytes. J Anim Poult Prod. 5:413–422.

Hammood AM, Aliawy ASh. 2017. Study on in vitro maturation of local buffalo oocytes. Life Sci Arch. 3:1082–1087.

Hasbi H, Sonjaya H, Gustina S. 2022. Oocytes population and development competence of Bali cattle embryo in vitro with different ovarian reproductive statuses. Trop Anim Sci J. 45:389–369. DOI:10.5398/tasj.2022.45.4.389.

Jamil H, Samad HA, Qureshi ZI, Rehman NU, Lodhi LA. 2008. Harvesting and evaluation of Riverine Buffalo follicular oocytes. Turk J Vet Anim Sci. 32:25–30.

Kandil O, Alhallag K, Shawky S, Abu-Alya I, Ismail E, Fathalla S. 2023. Effect of Seasons on Ovarian Activity and Developmental Competence of Buffalo Oocytes and Embryos. EJVS. 54:783–795. DOI:10.21608/ejvs.2023. 196836.1451.

Kumar S, Chaves M, da Silva A, Vale W, Filho S, Ferreira-Silva J, Melo L, Freita V. 2023. Factors affecting the in vitro embryo production in buffalo (Bubalus bubalis): A review. Vet Med (Praha). 68. DOI:10.17221/48/2022-VETMED.

Lemseffer Y, Terret M-E, Campillo C, Labrune E. 2022. Methods for assessing oocyte quality: A review of literature. Biomed. 10:2184. DOI:10.3390/biomedicines 10092184.

Lonergan P, Fair T. 2016. Maturation of oocytes in vitro. Annu Rev Anim Biosci. 4:255–268. DOI:10.1146/annurev-animal-022114-110822.

Mahesh YU, Rao MM, Sudhakar P, Rao KRSS. 2014. Effect of harvesting technique and presence or absence of corpus luteum on in vitro development after parthenogenetic activation of oocytes recovered from buffalo ovaries. Vet World. 7:315–320. DOI:10.14202/vetworld.2014.315-320.

Mansor MW. 2019. Effect of season and ovarian morphology of egyptian buffalo on oocyte quality and in vitro maturation rate. J Curr Vet Res. 1:34–41. DOI:10.21608 /jcvr.2019.11527.1005.

Mehmood A, Anwar M, Andrabi SMH, Afzal M, Naqvi SMS. 2011. In vitro maturation and fertilization of buffalo oocytes: the effect of recovery and maturation methods of recovery and maturation methods. Turk J Vet Anim Sci. 35:381–386. DOI:10.3906/vet-0812-17.

Mendes VRA, Costa EP, Queiroz VLD, Silva Júnior A, Alves SVP, Guimarães JD, Gomes LL. 2018. Impairment on nuclear maturation rate in oocytes from cows naturally infected by bovine herpesvirus 1 (BoHV-1). PVB. 38:2207–2212. DOI:10.1590/1678-5150-pvb-5860.

Mohammed AE-N, Al-Suwaiegh S, Al-Shaheen T. 2019. Effects of follicular fluid components on oocyte maturation and embryo development in vivo and in vitro. Adv Anim Vet Sci. 7. DOI:10.17582/journal.aav s/2019/7.5.346.355.

Nandi S, Raghu H, Ravindranatha B, Chauhan M. 2002. Production of buffalo (Bubalus bubalis) embryos in vitro: Premises and promises. Reprod Domest Anim. 37:65–74. DOI:10.1046/j.1439-0531.2002.00340.x.

Nikiforov D, Junping C, Cadenas J, Shukla V, Blanshard R, Pors SE, Kristensen SG, Macklon KT, Colmorn L, Ernst E, Bay-Bjørn A, Ghezelayagh Z, Wakimoto Y, Grøndahl ML, Hoffmann E, Andersen CY. 2020. Improving the maturation rate of human oocytes collected ex vivo during the cryopreservation of ovarian tissue. J Assist Reprod Genet. 37:891–904. DOI:10.1007/s10815-020-01724-7.

Ondho YS, Sutiyono, Setiatin ET, Sutopo utopo, Kurnianto E, Samsudewa D, Lestari DA, Setiaji A. 2024. Aspiration, slicing, and flushing medium techniques in collecting oocytes of sheep: searching for the best method. Online J Anim Feed Res. 14. DOI:10.51227/ojafr.2024.17.

Panda BSK, Pandey S, Somal A, Parmar MS, Bhat IA, Baiju I, Bharti MK, Sai Kumar G, Chandra V, Sharma GT. 2017. Leptin supplementation in vitro improved developmental competence of buffalo oocytes and embryos. Theriogenol. 98:116–122. DOI:10.1016/j.theriogenol ogy.2017.05.008

Pitroda MH, Khillare KP, Amle MB, Rangnekar MN, Meshram MD, Mali AB. 2021. Recovery of oocytes from ovarian follicles by aspiration method in buffaloes. J Entomol Zool Stud. 9:2124–2129.

Puri G, Chaudhary SS, Singh VK, Sharma AK. 2015. Effects of fetal bovine serum and estrus buffalo serum on maturation of buffalo (Bubalus bubalis) oocytes in vitro. Vet World. 8:143–146. DOI:10.14202/vetworld.2015 .143-146.

Rajendar M, Shah V, Bera S, Das S. 2024. Oocyte isolation techniques from the ovary samples of slaughtered animals: A review. Int J Adv Biochemist Res. 8:340–344. DOI:10.33545/26174693.2024.v8.i6Se.1307.

Rajesh K, Swathi B, Aruna Kumari G, Shanmugam M. 2018. Effect of ovarian status on oocyte quality and recovery rate retrieved by aspiration method in buffalo ovaries. IJCMAS. 7:1884–1889. DOI:10.20546/ijcmas.2018.704 .215.

Ravindranatha BM, Nandi S, Raghu HM, Reddy SM. 2003. Maturation and fertilization of buffalo oocytes: effects of storage of ovaries, IVM temperatures, storage of processed sperm and fertilization media. Reprod Domest Anim. 38:21–26. DOI:10.1046/j.1439-0531.2003.00390 .x.

Rose BI. 2014. Approaches to oocyte retrieval for advanced reproductive technology cycles planning to utilize in vitro maturation: a review of the many choices to be made. J Assist Reprod Genet. 31:1409–1419. DOI:10.1007/s10815-014-0334-9.

Ruhil S, Purohit GN. 2015. In vitro fertilization of buffalo oocytes matured in vitro in three different media. Indian J Anim Reprod. 36:10–13.

Saleh WM. 2017. Assessment of different methods of bovine oocytes collection, maturation and in vitro fertilization of abattoir specimens. Iraqi J Vet Sci. 31:55–65. DOI:10.33899/ijvs.2017.126711.

Sales JNS, Iguma LT, Batista RITP, Quintão CCR, Gama MAS, Freitas C, Pereira MM, Camargo LSA, Viana JHM, Souza JC, Baruselli PS. 2015. Effects of a high-energy diet on oocyte quality and in vitro embryo production in Bos indicus and Bos taurus cows. J Dairy Sci. 98:3086–3099. DOI:10.3168/jds.2014-8858.

Salzano A, Gasparrini B, Vecchio D, Longobardi V, Baruselli PS, Balestrieri A, Licitra F, D’Occhio M, Neglia G. 2019. Effect of photoperiod on follicular IGF-1 and oocyte quality independently of metabolic status in buffalo heifers. Ital J Anim Sci. 18:949–956. DOI:10.1080/ 1828051X.2019.1588793.

Segers I, Mateizel I, Van Moer E, Smitz J, Tournaye H, Verheyen G, De Vos M. 2015. In vitro maturation (IVM) of oocytes recovered from ovariectomy specimens in the laboratory: a promising “ex vivo” method of oocyte cryopreservation resulting in the first report of an ongoing pregnancy in Europe. J Assist Reprod Genet. 32:1221–1231. DOI:10.1007/s10815-015-0528-9.

Shabankareh HK, Shahsavari MH, Hajarian H, Moghaddam G. 2015. In vitro developmental competence of bovine oocytes: Effect of corpus luteum and follicle size. Iranian J Reprod Med. 13:615–622.

da Silva JCB, Alves MBR, Bridi A, Bohrer RC, Escobar GSL, de Carvalho JABA, Binotti WAB, Pugliesi G, Lemes KM, Chello D, Perecin F. 2021. Reproductive seasonality influences oocyte retrieval and embryonic competence but not uterine receptivity in buffaloes. Theriogenol. 170:77–84. DOI:10.1016/j.theriogenology.2021.04.019.

Singh WL, Sonowal J, Das A, Barua PM, Gogoi C, Mahanta D, Deuri N, Sathapathy S. 2018. Recovery of bovine oocytes in respect of quality and quantity by using different techniques. J Entomol Zool Stud. 6:250–253.

Sonowal J, Barua PM, Borah P, Dutta DJ, Hazarika G, Gogoi C, Deuri N, Das A, Borgohain I, Pathak B. 2017. Effect of ? -tocopherol and l-ascorbic acid on in- vitro maturation of vitrified bovine oocytes. Int J Chem Stud. 5:1359–1362.

Srirattana K, Hufana?Duran D, Atabay EP, Duran PG, Atabay EC, Lu K, Liang Y, Chaikhun?Marcou T, Theerakittayakorn K, Parnpai R. 2022. Current status of assisted reproductive technologies in buffaloes. Anim Sci J. 93. DOI:10.1111/asj.13767.

Udin Z, Masrizal M, Hendri H, Nanda S. 2020. Evaluation of different techniques in recovering of oocytes and storage duration of ovaries on the quality and quantity of bovine in vitro maturation. Bul Peternak. 44. DOI:10.21059/buletinpeternak.v44i2.52181.

Vuong LN, Le AH, Ho VNA, Pham TD, Sanchez F, Romero S, De Vos M, Ho TM, Gilchrist RB, Smitz J. 2020. Live births after oocyte in vitro maturation with a prematuration step in women with polycystic ovary syndrome. J Assist Reprod Genet. 37:347–357. DOI:10.1007/s10815-019-01677-6.

Xu H-Y, Yang X-G, Lu S-S, Liang X-W, Lu Y-Q, Zhang M, Lu K-H. 2018. Treatment with acetyl-l-carnitine during in vitro maturation of buffalo oocytes improves oocyte quality and subsequent embryonic development. Theriogenol. 118:80–89. DOI:/10.1016/j.theriogenology. 2018.05.033.

Yousaf MR, Chohan KR. 2003. Nuclear morphology, diameter and meiotic competence of buffalo oocytes relative to follicle size. Reprod Fertil Dev. 15:223. DOI:10.1071 /RD03006.