Supplementation of Black Soldier Fly (Hermetia illucens) on Activity and Capacity Phagocytic Macrophage of Laying Hens

Andri Cahya Irawan

Abstract

Black Soldier Fly are natural antibiotics. It is expected that the use of BSF larvae in poultry rations as an alternative source of conventional protein will contribute to improving the immune status and maintaining animal health, thereby reducing the use of antibiotic growth promoters (AGPs). This study aimed to evaluate the effect of the best type of BSF protein for determining the health status of laying hens based on the activity and capacity of macrophage phagocytosis on the non-protein A bacterium Staphyllococcus aureus. Results of this study indicated that the BSF extract (P3) has the highest phagocytic capacity value. This result proves that the BSF extract can induce macrophage cells to optimally process bacterial cells or foreign phagocyte particles. The highest average activity value, and phagocytic capacity of peritoneal macrophages was resulted from BSF extract (P3), respectively was 91.34 ± 0.38% and 22.84 macrophage-1 bacteria.

Keywords

Black Soldier Fly (BSF), antibiotic growth promoters (AGPs), activity and capacity of phagocytic peritoneal macrophages.

Full Text:

PDF

References

Abbas AK, Lichtman AH, Pilai S. 2017. Molecular and cellular immunology. 9th ed. Philadelphia (US): Elsevier.

Bosch G, Zhang S, Dennis GABO, Wouter HH. 2014. Protein quality of insects as potential ingredients for dog and cat foods. J Nutr Sci. 3:1-4.

Bovera F, Loponte R, Marono S, Piccolo G, Parisi G, Laconisi V, Gasco L, Nizza A. 2016. Use of tenebrio molitor larvae meal as protein source in broiler diet: effect on growth performance, nutrient digestibility, and carcass and meat traits. J anim Sci. 94: 639 – 647.doi:10.2527/jas2015-9201.

Choi WH, Yun JH, Chu JP, Chu KB. 2012. Antibacterial effects of extract of Hermetia illucens (Diptera: Stratiomydae) larvae against Gram-negative bacteria. Entomological Research. 42: 219-226.

Devereux G. 2002. The Immune System: An Overview. Calder PC, Field CJ, Gill

HS, editor. Oxon (UK): CABI Pub.

Diener S, Zurbrügg C, Tockner K. 2009. Conversion of organic material by BSF

larvae–Establishing optimal feeding rates. Waste Man & Res. 27: 603-610.

Erickson MC, Islam M, Sheppard C, Liao J, Doyle MP. 2004. Reduction of Escherichia coli O157:H7 and Salmonella enterica serovar Enteritidis in chicken manure by larvae of the Black Soldier Fly. J Food Prot. 67:685-690.

Finke MD. 2007. Estimate of chitin in raw whole insects. Zoo Biol. 26:105–115.

doi:10.1002/zoo.2012.

Finke, M.D. 2012. Complete nutrient content of four species of feeder insects Zoo Biology 00: 1–15.

Guyton AC, Hall JE. 2010. Textbook of Medical Physiology. 8th ed. Missisipi (US): Elsevier Inc.

Henry M, Gasco L, Piccolo G, Fountoulaki E. 2015. Review on the use of insects

in the diet of farmed fsh: Past and future. Anim Feed Sci Technol. 203:1–22. doi:10.1016/j.anifeedsci.2015.03.001.

Iswara LR. 2012. Introduction to Immunology. Medan (ID) : Universitas Sumatra Utara.

Kim, W., Bae, S., Park, K., Lee, S., Choi, Y., Han,S., Koh, Y. 2011. Biochemical characterization of digestive enzymes in the black soldier fly, Hermetia illucens (Diptera: Stratiomyidae). Journal of Asia-Pasific Entomology. 14 (1): 11-14. doi.org/10.1016/j.aspen.2010.11.003.

Kim SA, Rhee MS. 2016. Highly enhanced bactericidal effects of medium chain fatty acids (caprylic, capric, and lauric acid) combined with edible plant essential oil (carvacrol, eugenol, b-resorcylic acid, trans-cinnamaldehyde, thymol, and vanillin) against Escherichia coli O157:H7. Food Control. 60: 447 – 454. doi: 10.1016/j.foodcont.2015.08.022.

Klunder HC, Wolkers RJ, Korpela JM, Nout MJR. 2012. Microbiological aspect

of processing and storage of edible insects. Food Control. 26: 628-631.

Kroeckel, S., Harjes, A.G.E., Roth, I., Katz, H., Wuertz, S., Susenbeth, A. and Schulz, C., 2012. When a turbot catches a fly: evaluation of a pre-pupae meal of the black soldier fly (Hermetia illucens) as fish meal substitute Growth performance and chitin degradation in juvenile turbot (Psetta maxima). Aquaculture and Fisheries Management 364-365: 345-352.

Lalander C, Diener S, Magri ME, Zurbrugg C, Lindstrom A, Vinneras B. 2013. Faecal sludge management with the larvae of the Black Soldier Fly (Hermetia illucens)-from a hygiene aspect. Sci Total Enviroment. 458-460:312-318.

Liu Q, Tomberlin JK, Brady JA, Sanford MR, Yu Z. 2008. Black Soldier Fly (Diptera: Stratiomyidae) larvae reduce Escherichia coli in dairy manure. Environ Entomol. 37:1525-1530.

Makkar HPS, Tran G, Heuzé V, Ankers P. 2014. State of the art on use of insects

in animal feed. Journal Animal Feed Science. 197: 1–33. doi: 10.1016/j.anifeedsci.2014.07.008.

Mingyue L, Mohamedd AA, Zhang L, Zhang P, Zhang L. 2016. A family of CSab defensins and defensin-like peptides from the migrotory locustmigratoria, and their expression dynamics during mycosis and nosemosis. PLos One 11(8): e0161585.

Muhsinin M, Cece S. 2016. Association of NRAMP1 polymorphisms with immune traits in Indonesian native chickens. International journal of poultry science, 15 (10): 401-406. doi: 10.3923/ijps.2016.401.406.

Okti NP, Retno DS, Agustin I, Wibawan IWT. 2008. Peran antibodi kuning telur

(IgY) sebagai opsonion untuk pencegahan serangan mutan Streptococcus

serotipe D (Streptococcus sobrinus). Berkala Penelitian Hayati 13:129-134.

Perdigon G, Fuller R, Raya R. 2001. Lactic acid bacteria and their effect on the

immune system. Curr Issue in Intestinal Microbiol. 2: 27-42.

Rambet V, Umboh JF, Tulung YLR, Kowel YHS. 2016. Kecernaan protein dan energi ransum broiler yang menggunakan tepung maggot (Hermetia illucens) sebagai pengganti tepung ikan. J Zootek. 36:13-22.

Ravi C, Jeyashree A, Renuka DK. 2011. Antimicrobial peptides from inseks: an

overview. Rscrh in Biotech 2(5): 01-07.

Sánchez-Muros MJ, Barroso FG, Manzano-Agugliaro F. 2013. Insect meal as

renewable source of food for animal feeding: A review. J. Clean. Prod.

:16–27. doi:10.1016/j.jclepro.2013.11.068.

Steel RGD, Torrie JH. 1995. Prinsip dan Prosedur Statistika. Suatu Pendekatan

Biometrik. Terjemahan. 5th ed. Jakarta (ID): PT Gramedia Pustaka Utama.

St-Hilaire S, Cranfill K, McGuire MA, Mosley EE, Tomberlin JK, Newton L,

Sealey W, Sheppard C, Irving S. 2007. Fish offal recycling by the black soldier fly produces a foodstuff high in omega-3 fatty acids. J W Aquacult. Soc. 38: 309â€313.

Van Huis A, van Itterbeeck J, Klunder H, Mertens E, Halloran A, Muir G,

Vantome P. 2013. Edible Insects †Future Prospects for Food and Feed

Security. Rome (IT): FAO Forestry.

Villarroel AYR, Fortuny RS, dan Belloso OM. 2010. Natural antimicrobials for

food processing. Animal Sc Review : 211 – 217.

Wibawan IWT, Soejoedono RD. 2013. Intisari Imunologi Medis. Bogor (ID):

Fakultas Kedokteran Hewan IPB.

Refbacks

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.