Relationship of extender and packaging system an the length of preservation and the quality of chilled semen of Boer goat

Arie Febretrisiana; . Anwar; Simon Sinulingga

doi:10.14334/jitv.v21i1.1350

Relationship of extender and packaging system an the length of preservation and the quality of chilled semen of Boer goat

Arie Febretrisiana, . Anwar, Simon Sinulingga

DOI

https://doi.org/10.14334/jitv.v21i1.1350

Abstract

The aim of this research was to compare the effectiveness of different extender (either Triladyl or Tris Egg Yolk extender) and different packaging method (pool and straw) of chilled semen an the length of preservation and the quality of chilled semen of Boer goat. Semen was collected using an artificial vagina from 3 two years old Boer bucks with body weight of 50-55 kg. It was evaluated under a microscope, then each was diluted either in Tris egg yolk extender (TEY) or Triladyl. Those diluted sperms were then packed either in pool or straw and preserved at 5â°C refrigerator. Sperm motility, viability and membrane integrity of each group were evaluated every 24 h for up to 5 days. Results showed that sperm motility in Triladyl ofÂ pool packaging system up to 3 days was higher than straw packaging system or TEY in pool or straw packaging system which were 45.8%, 26.1%, 32.1% and 9.1%, respectively (P<0.05). Percentage of sperm membrane integrity showed the same pattern to Triladyl both in pool and straw packaging system which was higher than TEY group (75.2% and 77,2%; P<0.05). Sperm viability in Triladyl both in pool or straw packaging system decreased (P<0.05) after 3 days of preservation (77.1% and 76.2%) but TEY significanly decreased after 4 days of preservation either in pool or straw packaging system (73.2% and 58.0%; P<0.05). It was concluded that sperm quality decreased with increasing of the length of preservation while Triladyl extender in pool packaging system showed the best quality.

Key Words: Chilled Semen, Boer, Triladyl, Tris Egg Yolk, Straw

Keywords

Chilled Semen; Boer; Triladyl; Tris Egg Yolk; Straw

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References

Ariantie OS, Yusuf TL, Sajuthi D, Arifiantiny RI. 2014. Kualitas semen cair kambing Peranakan Etawah dalam modifikasi pengencer tris dengan trehalosa dan rafinosa. J Vet. 15:11-22.

Arifiantini RI, Purwantara B. 2010. Motility and viability of Fresian Holstein spermatozoa in three different extender preserved at 5Â°C. J Indones Trop Anim Agric. 35:222-226.

Alves HM, Oliveira IR, Castelo TS, Lima GL, Souza AL, Moreira AL, de Paula VV, Silva AR. 2013. Comparison of different glycerol and egg yolk concentrations added to tris-based extender for the collared peccaries (Tayassu tajacu) semen freezing. Reprod Domest Anim. 48:506-511.

Botham KM, Mayes PA. 2009. Pengangkutan dan penyimpanan lipid. In: Murray RK, Granner DK, Rodwell VW, editors. Harperâ€™s Illustrated Biochemistry. 27th ed. Pendit BU, penerjemah. Jakarta (Indones): EGC. p. 225-238.

Holt WV, Abaigar T, Jabbour HN. 1996. Oestrus synchronization, semen preservation and artificial insemination in the Mohor Gazelle (Gazella dama mhorr) for the establishment of a genome resource bank programme. Reprod Fertil Dev. 8:1215-1222.

Ismaya. 2014. Bioteknologi inseminasi buatan pada sapi dan kerbau. Yogyakarta (Indones): Gadjah Mada University Press.

Klinc P, Rath. 2006. Aplication of flowcytometrically sexed spermatozoa in different farm animal species: a review. Archiv Tierzuch Dummerstorf. 49:41-54

Kasimanicham VR, Kasimanicham RK, Memon MA. 2012. Effect of extenders on sperm mitcondrial membrane, plasma membrane and sperm kinetics during liquid preservation of canine semen at 5Â°C. J Amin Reprod Sci. 136:139-145.

Leboeuf B, Restall B, Salamon S. 2000. Production and storage of goat semen for artificial insemination. Anim Reprod Sci. 62:113-141.

Leethongdee S. 2010. Development of trans-cervical artificial Insemination in sheep with special reference to anatomy of cervix. Suranaree J Sci Technol. 17:157-169.

Love CC, Thompson JA, Brinsko SP, Rigby SL, Blanchard TL, Lowry VK, Varner DD. 2003. Relationship between stallion sperm motility and viability as detected by two fluorescence staining techniques using flow cytometry. Theriogenology. 60:1127-1138.

Mara L, Dattena M, Pilichi S, Sanna D, Branca A, Cappai P. 2007. Effect of different extender on sperm motility: collection and goat semen fertility, evaluation of semen. Anim Reprod Sci. 102:152-157.

Medrano A, Garcia-Gill N, Ramio L, Montserrat RM, Pinart E, Concha II, Bonet S, Rigau T, Rodriguez-Gill JE. 2006. Hexose-specificity of hexokinase and ADP-dependence of pyruvate kinase play important roles in the controlof monosaccaride utilization in freshly diluted boar spermatozoa. Mol Reprod Dev. 73:1179-1194.

Munsi MN, Buiyan MMU, Alam MGS. 2007. Effects of exogenous gluthathione on the quality of chilled bull semen. Reprod Domest Anim. 42:358-362

Paulenz H, Soderquist L, Perez R, Berg KA. 2009. Effect of different extenders and sorage temperature on sperm viability of liquid ram semen. Theriogenology. 57:823-836.

Revell SG, Mrode RA. 1994. An osmotic resistance test for bovine semen. Anim Reprod Sci. 36:77-86.

Salomon S, Maxwell WMC. 2000. Preservation of ram semen. Anim Reprod Sci. 62:77-111.

Stefanov RG, Anev G, Abadjieva DV. 2015. Effect of different extenders and strage periods on motility and fertility of ram sperm. Mac Vet Rev. 38:85-89.

Schorin, Marilyn D, Sollid, Kris RD, Edge, Marianne MS, Bouchoux, Ann MSW. 2012. The science of sugar part 1: A closer look at sugars. Nurt Today. 3:96-101.

Taylor MJ. 2006. Biology of cell survival in the cold: The basis for biopreservation of tissues and organs. CRC-Taylor & Francis. p. 15-62.

Verberckmoes S, Van Soom A, Dewulf J, De Pauw I, de Kruif A. 2004. Preservation of fresh bovine semen in extender based on the ionic composition of cauda epydidimal plasma. Reprod Domest Anim. 39:1-7.