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The Quality of Chilled Local Ram Semen After a Rest Period from the Breeding Program
Abstract
Semen quality is influenced by various factors, including sexual rest. This study aimed to evaluate the quality of chilled semen from Indonesian local rams subjected to sexual rest. Semen was collected from four rams using an artificial vagina and evaluated macroscopically (volume, colour, consistency, pH, and mass movement) and microscopically (sperm concentration, progressive motility, viability, and abnormality). Semen was then diluted using either Tris-fructose-egg yolk (Tris) or sodium citrate-fructose-egg yolk (Na-citrate) extenders to a concentration of 50×10? cells/0.2 ml. The diluted semen was stored in sealed centrifuge tubes at 4–5°C. Progressive motility and viability of spermatozoa in chilled semen were assessed every 24 hours for four days. The influence of scrotal circumference (SC) on both fresh and chilled semen quality was also evaluated. Results showed that fresh semen had a volume of 1.08±0.12 mL, milky white colour, thin to thick consistency, pH of 6.42±0.13, mass movement score of 2.94±0.13, concentration of 4941±926.84×10?/mL, progressive motility of 79.38±2.17%, viability of 87.74±3.48%, and abnormality of 7.99±3.14%. Sexual rest increased sperm concentration and abnormality, while SC significantly influenced progressive motility, viability, concentration, and abnormality in fresh semen. However, sexual rest had no significant effect on chilled semen quality. The Tris extender could maintain sperm motility and viability significantly better (P < 0.05) than the Na-citrate extender. Additionally, chilled semen quality was influenced by SC. In conclusion, sexual rest improved certain parameters of fresh semen quality but did not significantly affect the quality of chilled semen in local Indonesian rams.
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References
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