The Impact Addition Egg Yolk In Various Concentration Into The Tyrode Extender To Muscovy Duck (Cairina Moschata) Sperm Quality

Siti Darodjah Rasad; Rini Widyastuti; Iwan Setiawan; Endang Sujana; Rangga Setiawan; Nurcholidah Solihati; Mohd. Noor Hisham Mohd. Nadzir

doi:10.14334/jitv.v30i2.3513

The Impact Addition Egg Yolk In Various Concentration Into The Tyrode Extender To Muscovy Duck (Cairina Moschata) Sperm Quality

Siti Darodjah Rasad, Rini Widyastuti, Iwan Setiawan, Endang Sujana, Rangga Setiawan, Nurcholidah Solihati, Mohd. Noor Hisham Mohd. Nadzir

DOI

https://doi.org/10.14334/jitv.v30i2.3513

Abstract

This study investigated at how different concentrations of egg yolk in Tyrode extender impacted the quality of Muscovy duck semen after preservation. A sample of semen was collected from 1.5-year-old Muscovy ducks. The sample was evaluated for sperm motility, plasma membrane integrity, acrosome integrity, and sperm morphology before being separated into three equal parts and diluted in Tyrode extender containing increasing levels of egg yolk: T₁ (5%), T₂ (10%), and T₃ (15%). After 21 hours of preservation at 4-5ºC, each sample was evaluated for the same parameters as fresh semen. The study found a significant decrease in sperm motility, membrane integrity, and acrosome integrity following preservation in T₁, T₂, and T₃ compared to fresh ejaculate (53.34%; 63.5%; 60.04% vs 84.16%), (48.7%; 57.8%; 55.3% vs. 85.6%), and (50.6%; 57.6%; 56.1% vs 85.4%), respectively (p<0.05). In contrast, abnormal sperm morphology increased considerably in T₁, T₂, and T₃ compared to fresh semen (6.69%, 6.89%, 7.89% vs 2.2%) (p < 0.05). Furthermore, the addition of 10% egg yolk to the Tyrode extender resulted in the best percentages of sperm motility, sperm membrane integrity, and acrosome integrity following preservation. Surprisingly, the percentage of sperm with abnormal morphology did not differ significantly (p > 0.05) between T₁, T₂, and T₃ after the preservation period. It can be concluded that adding 10% egg yolk to the extender substantially improved sperm motility, membrane integrity, and acrosome integrity in Muscovy duck sperm after 21 hours of preservation.

Keywords

egg yolk, sperm quality, preservation, Thyrode

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