CSN1S1 Gene Polymorphism of Indonesian Local PE, Saanen, and Sapera Goats

Ega Anggraini; Anneke Anggraeni; Cece Sumantri; Afton Atabany

doi:10.14334/jitv.v30i1.3470

CSN1S1 Gene Polymorphism of Indonesian Local PE, Saanen, and Sapera Goats

Ega Anggraini, Anneke Anggraeni, Cece Sumantri, Afton Atabany

DOI

https://doi.org/10.14334/jitv.v30i1.3470

Abstract

CSN1S1, or alpha-s1 casein, gene is one of milk's four significant casein-controlling genes. Genetic variations in this gene have been proposed to affect milk protein quality in dairy goats. This study investigated the genetic polymorphism of the CSN1S1 gene using a direct sequencing method in local Peranakan Etawah (PE), exotic Saanen, and their cross or Sapera goats. Blood samples were collected from 66 Sapera, 15 Saanen, and 14 PE does at the Indonesian Research Institute for Animal Production (IRIAP) in Bogor, West Java Province. The MEGA11 program was used to analyze the sequence data of the CSN1S1 gene in DNA fragment exon 12 and partial intron 12. A Popgen 3.2 packet program was applied to analyze allele and genotype frequencies, Hardy-Weiberg (H-W) equilibrium, and the degree of heterozygosity (Ho) values. Four SNPs were identified: g.10243 G>A, g.10250 A>G, g.10277 G>A, and g.10283 T>G. Based on the allele and genotype frequencies, H-W equilibrium, and Ho values, the CSN1S1 gene at the four loci was polymorphic in all three goat genotypes. These SNPs may be considered candidate selection markers for milk protein quality. This study provides insights into the genetic diversity of CSN1S1, which is crucial for molecular selection to improve milk protein quality in local PE and its crosses.

Keywords

CSN1S1 Gene; Dairy Goat; SNP

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