Study on Animal Welfare of Sheep Transported with Methionine Hydroxy Analog and Dextrose Supplementation

Muhamad Baihaqi; Henny Nuraini; Anuraga Jayanegara; Wasmen Manalu

doi:10.14334/jitv.v29i3.3449

Study on Animal Welfare of Sheep Transported with Methionine Hydroxy Analog and Dextrose Supplementation

Muhamad Baihaqi, Henny Nuraini, Anuraga Jayanegara, Wasmen Manalu

DOI

https://doi.org/10.14334/jitv.v29i3.3449

Abstract

Livestock transportation has adversely effects on animal welfare and performance during subsequent production periods. This research aimed to evaluate the effects of methionine hydroxy analogue (MHA) and dextrose (additive) supplementations before transportation on the animal welfare status of sheep. The study used 27 thin-tailed male sheep with an average body weight of 21,8±2,0 kg, 10-12 months of age, which were divided into three treatments: sheep without transportation and additive supplementation (K: Control); sheep transported without additive supplementation (P1); sheep transported with additive supplementation (P2: MHA 5 mg/kg body weight and dextrose 5 mg/kg body weight). Sheep in groups P1 and P2 were transported for 8 hours journey using commercial vehicles that usually used by local farmers. The results showed that sheep in the P2 group significantly had lower body weight loss compared to those in P1 group and had a faster recovery time (P<0.05). Sheep in the P2 group also demonstrated better post-transportation behaviour than those in P1, with no significant difference from the control group. The experimental sheep in P2 group had a significantly lower N/L ratio than those in P1 and was comparable to the control group (P<0.05). The P2 group also has significantly lower cortisol hormone and blood glucose concentrations than P1, which indicates an improvement in sheep welfare status in P2 and Control groups. Therefore, it can be concluded that supplementation of MHA and dextrose before departure improves sheep's welfare status under transportation.

Keywords

Animal Welfare, Dextrose, Methionine hydroxy analogue, Sheep

References

Aasif Shahzad M, Nisa M, Sarwar M. 2011. Influence of replacing corn grain by enzose (corn dextrose) on nutrient utilization, thyroid hormones, plasma metabolites, and weight gain in growing lambs. Asian-Australas J Anim Sci. 24(7):946–951. https://doi.org/10.5713/ajas.2011.10407

Ahmad Mir N, Ashutosh A, Ahmad Shergojry S, Ahmed Wani S, Ahmad Sheikh F. 2019. Effect of induced transportation stress in goats supplemented with vitamin C and jaggery during hot dry season. Biol Rhythm Res. 50(3):389–399. https://doi.org/10.1080/09291016.2018.1452591

Baihaqi M, Dekrityana L, Putra S. 2017. Performa dan respon fisiologi domba ekor gemuk pada lama transportasi yang berbeda. https://fapet.ipb.ac.id/snip/pdf/proceeding_snip.pdf.:198–201.

Baihaqi M, Nuraini H, Jayanegara A, Manalu W. 2022. Effect of methionine hydroxy analog (MHA) or dextrose supplementation on physiological responses and behaviours of sheep under transportation stress. In: IOP Conf Ser Earth Environ Sci. Vol. 1114. [place unknown]: Institute of Physics. https://doi.org/10.1088/1755-1315/1114/1/012085

Biobaku KT, Omobowale TO, Akeem AO, Aremu A, Okwelum N, Adah AS. 2018. Use of goat interleukin-6, cortisol, and some biomarkers to evaluate clinical suitability of two routes of ascorbic acid administration in transportation stress. Vet World. 11(5):674–680. https://doi.org/10.14202/vetworld.2018.674-680

Carbillet J, Rey B, Lavabre T, Chaval Y, Merlet J, Débias F, Régis C, Pardonnet S, Duhayer J, Gaillard JM, et al. 2019. The neutrophil to lymphocyte ratio indexes individual variation in the behavioural stress response of wild roe deer across fluctuating environmental conditions. Behav Ecol Sociobiol. 73(11). https://doi.org/10.1007/s00265-019-2755-z

Department of Agriculture and Water Resources. 2023. Review of the Australian Standards for the Export of Livestock. https://www.agriculture.gov.au/sites/default/files/sitecollectiondocuments/animal/review-asel-sea-transport-final-report.pdf.

Fraser A, Broom D. 1997. Farm animal behaviour and welfare. 3rd ed. Wallingford: CAB International.

Gasparino E, Del Vesco AP, Khatlab AS, Zancanela V, Grieser DO, Silva SCC. 2018. Effects of methionine hydroxy analogue supplementation on the expression of antioxidant-related genes of acute heat stress-exposed broilers. Animal. 12(5):931–939. https://doi.org/10.1017/S1751731117002439

Gupta D, Kashyap G, Ashutosh M, Ashutosh. 2020. Ameliorative effect of Vitamin C, Electrolyte and Jaggery on Transportation Stress at different flocking densities in Hot Humid and Winter Seasons on Hormonal parameters of goats. Livest Sci. 242:104271. https://doi.org/10.1016/J.LIVSCI.2020.104271

Jacometo CB, Zhou Z, Luchini D, Trevisi E, Corrêa MN, Loor JJ. 2016. Maternal rumen-protected methionine supplementation and its effect on blood and liver biomarkers of energy metabolism, inflammation, and oxidative stress in neonatal Holstein calves. J Dairy Sci. 99(8):6753–6763. https://doi.org/10.3168/JDS.2016-11018

Lendrawati. 2020. Evaluasi Pengangkutan, Suplementasi Pakan, dan Desain Kontainer untuk Meningkatkan Kesejahteraan Domba selama Transportasi [Dissertation]. Bogor: IPB University.

Lendrawati L, Priyanto R, Jayanegara A, Manalu W, Desrial D. 2020. Effect of different transportation period on body weight loss, hematological and biochemical stress responses of sheep. J Indones Trop Anim Agric. 45(2):115–123. https://doi.org/10.14710/jitaa.45.2.115-123

Martínez Y, Li X, Liu G, Bin P, Yan W, Más D, Valdivié M, Hu CAA, Ren W, Yin Y. 2017. The role of methionine on metabolism, oxidative stress, and diseases. Amino Acids. 49(12):2091–2098. https://doi.org/10.1007/s00726-017-2494-2

Miranda-de la Lama GC, Salazar-Sotelo MI, Pérez-Linares C, Figueroa-Saavedra F, Villarroel M, Sañudo C, Maria GA. 2012. Effects of two transport systems on lamb welfare and meat quality. Meat Sci. 92(4):554–561. https://doi.org/10.1016/J.MEATSCI.2012.05.026

Miranda-de la Lama GC, Villarroel M, María GA. 2014. Livestock transport from the perspective of the pre-slaughter logistic chain: a review. Meat Sci. 98(1):9–20. https://doi.org/10.1016/J.MEATSCI.2014.04.005

Mousaie A, Valizadeh R, Naserian AA, Heidarpour M, Mehrjerdi HK. 2014. Impacts of Feeding Selenium-Methionine and Chromium-Methionine on Performance, Serum Components, Antioxidant Status, and Physiological Responses to Transportation Stress of Baluchi Ewe Lambs. Biol Trace Elem Res. 162(1–3):113–123. https://doi.org/10.1007/s12011-014-0162-x

Navarro G, Col R, Phillips CJC. 2018. Effects of space allowance and simulated sea transport motion on behavioural and physiological responses of sheep. Appl Anim Behav Sci. 208:40–48. https://doi.org/10.1016/J.APPLANIM.2018.08.009

Pascual-Alonso M, Miranda-de la Lama GC, Aguayo-Ulloa L, Villarroel M, Mitchell M, María GA. 2017. Thermophysiological, haematological, biochemical and behavioural stress responses of sheep transported on road. J Anim Physiol Anim Nutr (Berl). 101(3):541–551. https://doi.org/10.1111/jpn.12455

Pastell M. 2016. CowLog – Cross-Platform Application for Coding Behaviours from Video. J Open Res Softw. 4(1):15. https://doi.org/10.5334/jors.113

Raghazli R, Othman AH, Kaka U, Abubakar AA, Imlan JC, Hamzah H, Sazili AQ, Goh YM. 2021. Physiological and electroencephalogram responses in goats subjected to pre-and during slaughter stress. Saudi J Biol Sci. 28(11):6396–6407. https://doi.org/10.1016/J.SJBS.2021.07.013

Rey-Salgueiro L, Martinez-Carballo E, Fajardo P, Chapela MJ, Espiñeira M, Simal-Gandara J. 2018. Meat quality in relation to swine well-being after transport and during lairage at the slaughterhouse. Meat Sci. 142:38–43. https://doi.org/10.1016/J.MEATSCI.2018.04.005

Sauls-Hiesterman JA, Banuelos S, Atanasov B, Bradford BJ, Stevenson JS. 2020. Physiologic responses to feeding rumen-protected glucose to lactating dairy cows. Anim Reprod Sci. 216:106346. https://doi.org/10.1016/J.ANIREPROSCI.2020.106346

Townshend A, Poole CF, Miró M, Worsfold P. 2019. Encyclopedia of Analytical Science. Amsterdam: Elsevier.

Del Vesco AP, Gasparino E, De Oliveira Grieser D, Zancanela V, Soares MAM, De Oliveira Neto AR. 2015. Effects of methionine supplementation on the expression of oxidative stress-related genes in acute heat stress-exposed broilers. British Journal of Nutrition. 113(4):549–559. https://doi.org/10.1017/S0007114514003535

Willemsen H, Swennen Q, Everaert N, Geraert PA, Mercier Y, Stinckens A, Decuypere E, Buyse J. 2011. Effects of dietary supplementation of methionine and its hydroxy analog dl-2-hydroxy-4-methylthiobutanoic acid on growth performance, plasma hormone levels, and the redox status of broiler chickens exposed to high temperatures. Poult Sci. 90(10):2311–2320. https://doi.org/10.3382/PS.2011-01353

Wu Y, Guo X, Zhao D, Xu C, Sun H, Yang Q, Wei Q, Si H, Wang K, Zhang T. 2022. Effect of Methionine Supplementation on Serum Metabolism and the Rumen Bacterial Community of Sika Deer (Cervus nippon). Animals. 12(15). https://doi.org/10.3390/ani12151950

Xu K, Yang K, Yang Y, Wu W, Zhou C. 2023. Supplementation of feed and water after long-duration road transportation: The efects on welfare and rumen fermentation in goats. Front Vet Sci. 10:1–8.