Optimal and Tolerant Conditions for Alginate and Calcium Chloride for the Semen Encapsulation of Pasundan Bull

Ayesha Khan; Muhammad Abdul Manan; Daud Samsudewa; Fitra Aji Pamungkas; Hamza Zulfiqar; Saad Irfan; Kiran Haidari; Vidi Wulandari; Didik Nurul Hadi

doi:10.14334/jitv.v29i2.3430

Optimal and Tolerant Conditions for Alginate and Calcium Chloride for the Semen Encapsulation of Pasundan Bull

Ayesha Khan, Muhammad Abdul Manan, Daud Samsudewa, Fitra Aji Pamungkas, Hamza Zulfiqar, Saad Irfan, Kiran Haidari, Vidi Wulandari, Didik Nurul Hadi

DOI

https://doi.org/10.14334/jitv.v29i2.3430

Abstract

The study aimed to determine the tolerance of Pasundan bull's sperm with different alginate and calcium chloride (CaCl2) concentrations to identify optimal conditions for sperm encapsulation. Semen samples were collected weekly with artificial vaginas from pasundan bulls. Pooled semen was divided into 9 equal volumes. The first sample was diluted with tris egg yolk extender (control), 4 samples were diluted with tris egg yolk extender supplemented with different concentrations of alginate (0.25, 0.5, 0.75, and 1%), and 4 other samples with CaCl2 (2.5, 5, 10, and 20 mM). Evaluation of semen was determined after 5 min incubation at room temperature and after overnight storage at 5 °C. Results showed that after 24h of refrigerated storage, the values for total motility and progressive motility in the groups with 2.5 mM CaCl2 and 0.25% alginate showed no difference compared to the control group. In contrast, a significant difference (P<0.05) was found between the other groups with CaCl2 and alginate. The progressive motility value in the group with alginate concentration greater than 0.25% decreased significantly (P<0.05). There was no difference between the groups (both alginate and CaCl2) in the spermatozoa viability and plasma membrane integrity variable. In conclusion, sperm with 2.5 mM calcium chloride and 0.25 % alginate was more tolerant of appropriate prolonged exposure and the sperm encapsulation process.

Keywords

Alginate, calcium chloride, sperm tolerance, encapsulation, pasundan bull semen

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