Diversity of the Monoamine Oxidase A Gene in Beef Cattle

Nadira Andarini Siregar; Ronny Rachman Noor; Mokhamad Fakhrul Ulum; Jakaria Jakaria

doi:10.14334/jitv.v29i3.3414

Diversity of the Monoamine Oxidase A Gene in Beef Cattle

Nadira Andarini Siregar, Ronny Rachman Noor, Mokhamad Fakhrul Ulum, Jakaria Jakaria

DOI

https://doi.org/10.14334/jitv.v29i3.3414

Abstract

Monoamine Oxidase A (MAOA) is a gene that controls aggressive traits. The MAOA gene plays a role in encoding the monoamine oxidase A enzyme, which plays a role in the catabolism of neurotransmitters, including dopamine, norepinephrine, and serotonin. This study aims to identify the diversity of the MAOA SNP gene in beef cattle using sequencing methods. This research used 127 cattle DNA samples to identify diversity, including Bali, Limousin, Wagyu, PO, Madura, and Wagyu-Bali (F1) cattle. MAOA gene polymorphisms in the promoter and exon 1 were analyzed using sequencing methods. Genotype frequencies, allele frequencies, heterozygosity values, and Hardy-Weinberg balance were calculated using the PopGen32 program. The results showed that the MAOA gene in the promoter region has six SNPs, one of which is SNP g.385G>A, while the MAOA gene in exon 1 is monomorphic. The PCR-RFLP method was used to investigate the SNP g.385G>A MAOA gene polymorphism using the RSaI restriction enzyme. The MAOA gene was detected in 3 genotypes: GG, GA, and AA. SNP g.385G>A is polymorphic in Bali, PO, Madura, and Wagyu-Bali cross (F1) cattle while monomorphic in Limousin and Wagyu cattle. Further studies are necessary to explore the functional implications of SNP g.385G>A and their relationship to aggressive behaviors in cattle.

Keywords

Beef Cattle; Genetic Diversity; MAOA Gene; Single Nucleotide Polymorphism

References

Adinata Y, Noor RR, Priyanto R, Cyrilla L, Sudrajad P. 2023. Morphometric and physical characteristics of Indonesian beef cattle. Arch Anim Breed. 66:153–161. DOI:10.5194/aab-66-153-2023.

Amiano K, Busono W, Maylinda S. 2020. Productivity of Bali cattle on Peatland in Central Kalimantan. J Trop Anim Prod. 21:39–43. DOI:10.21776/ub.jtapro.2020.021.01.4.

Anwar S, Putra AC, Wulandari AS, Agung PP, Putra WPB, Said S. 2017. Genetic polymorphism analysis of 5' untranslated region of thyroglobulin gene in Bali cattle (Bos javanicus) from three different regions of Indonesia. J Indones Trop Anim Agric. 42:175–184. DOI:10.14710/jitaa.42.3.175-184.

Chen R, Chu Q, Shen C, Tong X, Gao S, Liu X, Zhou B, Schinckel AP. 2019. Identification of single nucleotide polymorphisms in porcine MAOA gene associated with aggressive behavior of weaned pigs after group mixing. Animals. 9. DOI:10.3390/ani9110952.

Cooke RF. 2014. Bill E. Kunkle Interdisciplinary Beef Symposium: Temperament and acclimation to human handling influence growth, health, and reproductive responses in Bos taurus and Bos indicus cattle. J Anim Sci. 925325–5333. DOI:10.2527/jas.2014-8017.

Dairoh D, Jakaria J, Ulum MF, Sumantri C. 2022. A New SNPs at 3’UTR Region of calpain 1 gene and its association with growth and meat quality traits in beef cattle. J Indones Trop Anim Agric. 47:17–28. DOI:10.14710 /jitaa.47.1.17-28.

Edgnülü TG, Özge A, Erdal N, Kuru O, Erdal ME. 2014. Association analysis of the functional MAO a gene promoter and MAOB gene intron 13 polymorphisms in tension-type headache patients. Adv Clin Exp Med. 23:901–906.DOI:10.17219/acem/37333.

Eusebi PG, Cortés O, Carleos C, Dunner S, Cañon J. 2018. Detection of selection signatures for agonistic behaviour in cattle. J Anim Breed Genet. 135:170–177. DOI:10.1111/jbg.12325.

Eusebi PG, Sevane N, Cortés O, Contreras E, Cañon J, Dunner S. 2020. Aggressive behavior in cattle is associated with a polymorphism in the MAOA gene promoter. Anim Genet. 51:14–21. DOI:10.1111/age.12867.

Eusebi PG, Sevane N, O'Rourke T, Pizarro M, Boeckx C, Dunner S. 2021. Gene expression profiles underlying aggressive behavior in the prefrontal cortex of cattle. BMC Genomics. 22:1–14. DOI:10.1186/s12864-021-07505-5.

Ferreira AR, Aguiar Filho LFC, Sousa R V., Sartori R, Franco MM. 2015. Characterization of allele-specific expression of the X-linked gene MAO-A in trophectoderm cells of bovine embryos produced by somatic cell nuclear transfer. Genet Mol Res. 14:12128–12136. DOI:10.4238/2015.October.5.26.

Handiwirawan EKO. 2012. Identifikasi Single Nucleotide Polymorphism pada Gen Mono Amine Oxidase A sebagai Penanda Genetik untuk Sifat Agresif pada Domba. 17:258–275.

Hashim HO, Al-Shuhaib MBS. 2019. Exploring the potential and limitations of PCR-RFLP and PCR-SSCP for SNP detection: A review. J Appl Biotechnol Reports. 6:137–144. DOI:10.29252/JABR.06.04.02.

Ismail R, Handiwirawan E, Elieser S, Jakaria J. 2020. Polymorphism of 5’UTR myostatin gene indel (g.1256/TTTTA) and its association with body weight in Boerka crossbred goat. J Indones Trop Anim Agric. 45:163–172.DOI:10.14710/jitaa.45.3.163-172.

Jakaria J, Kholijah K, Darwati S, Rahman Q, Daulay WL, Suhendro I, Londra IM, Ulum MF, Noor RR. 2023. Lack of association between coat color abnormalities in Bali cattle (Bos javanicus) and the coding regions of the MC1R and KIT genes. Vet World. 16:1312–1318. DOI:10.14202/vetworld.2023.1312-1318.

Karimah SN, Noor RR, Jakaria J. 2021. Identification of SNP g.10428C>T of Stearoyl-CoA Desaturase gene related to meat quality in Bali cattle using PCR-RFLP method. J Indones Trop Anim Agric. 46:295–303. DOI:10.14710 /jitaa.46.4.295-303.

Khasanah H, Gunawan A, Priyanto R, Ulum MF, Jakaria. 2016. Polymorphism of Myostatin (MSTN) promoter gene and its association with growth and muscling traits in Bali cattle. Media Peternak. 39:95–103. DOI:10.5398/medpet .2016.39.2.95.

Laya NK, Arifin MA, Syahruddin S, Fathan S, Datau F, Gubali SI, Dako S. 2024. Characteristics of Male Bali cattle in Toili District. Jambura J Anim Sci. 6:145–151. DOI:10. 35900/jjas.v6i2.22174.

Li YX, Jin HG, Yan CG, Seo KS, Zhang LC, Ren CY, Jin X. 2013. Association of CAST gene polymorphisms with carcass and meat quality traits in Yanbian cattle of China. Mol Biol Rep. 40:1875–1881. DOI:10.1007/s11033-012-2243-2.

Lindahl C, Pinzke S, Herlin A, Keeling LJ. 2016. Human-animal interactions and safety during dairy cattle handling-Comparing moving cows to milking and hoof trimming. J Dairy Sci. 99:2131–2141. DOI:10.3168/j ds.2014-9210.

Lühken G, Glenske K, Brandt H, Erhardt G. 2010. Genetic variation in monoamine oxidase A and analysis of association with behaviour traits in beef cattle. J Anim Breed Genet. 127:411–418. DOI:10.1111/j.1439-0388. 2010.00855.x.

Peden RSE, Akaichi F, Camerlink I, Boyle LA, Turner SP. 2019. Factors influencing farmer willingness to reduce aggression between pigs. Animals. 9. DOI:10.33 90/ani9010006.

Pratiwi N, Maskur, Priyanto R, Jakaria. 2016. Novel snp of calpain-1 (CAPN1) gene and its association with carcass and meat characteristics traits in Bali cattle. J Indones Trop Anim Agric. 41:109–116. DOI:10.14710/jitaa.41 .3.109-116.

Putra IGR, Sari DA, Rachmawati SM, Oktaviani R, Noor RR, Jakaria J. 2021. Identification of single nucleotide polymorphism c.957A>C of PLAG1 gene and its association with growth traits in Bali cattle. J Indones Trop Anim Agric. 46:199–208. DOI:10.14710/jitaa.46.3. 199-208.

Putri RF, Nugraha CD, Furqon A, Septian WA, Suyadi S. 2021. Identifikasi Keragaman Genetik Gen Inhibin Subunit Alpha (INHA) dan Inhibin Subunit Beta A (INHBA) Pada Sapi Peranakan Ongole (PO). J Trop Anim Prod. 22:69–79. DOI:10.21776/ub.jtapro.2021.022.01.9.

van Rhijn JR, Shi Y, Bormann M, Mossink B, Frega M, Recaioglu H, Hakobjan M, Klein Gunnewiek T, Schoenmaker C, Palmer E, et al. 2022. Brunner syndrome-associated MAOA mutations result in NMDAR hyperfunction and increased network activity in human dopaminergic neurons. Neurobiol Dis. 163:105587. DOI:10.1016/j.nbd.2021.105587.

Sacco J, Ruplin A, Skonieczny P, Ohman M. 2017. Polymorphisms in the canine monoamine oxidase a (MAOA) gene: identification and variation among five broad dog breed groups. Canine Genet Epidemiol. 4:1–8. DOI:10.1186/s40575-016-0040-2.

Sari DAP, Muladno, Said S, Nahrowi, Priyanto R. 2021. Performance of female Bali cattle in different management systems at Field Station of Sekolah Peternakan Rakyat (SL-SPR) Kuamang Abadi. IOP Conf Ser Earth Environ Sci. 888. DOI:10.1088/1755-1315/888/1/012006.

Setyani NMP, Priyanto R, Jakaria J. 2021. The Effect of SNP c.100800G > A on CAST|Cfr13I Gene Polymorphisms with Ultrasound Imaging of Meat Characteristics and Growth Traits in Bali Cattle. Iran J Appl Anim Sci. 11:707–715.

Titterington FM, Knox R, Morrison SJ, Shirali M. 2022. Behavioural traits in Bos taurus Cattle, their heritability, potential genetic markers, and associations with production traits. Animals. 12. DOI:10.3390/ani1 2192602.

V R, Husain RS A. 2017. MAOA Gene Associated with Aggressive Behavior in Humans. J Down Syndr Chromosom Abnorm. 03:1–3. DOI:10.4172/2472-1115 .1000120.

Yen JY, Chou WP, Lin HC, Wu HC, Tsai WX, Ko CH. 2021. Roles of hostility and depression in the association between the maoa gene polymorphism and internet gaming disorder. Int J Environ Res Public Health. 18. DOI:10.3390/ijerph18136910.

Ziegler C, Domschke K. 2018. Epigenetic signature of MAOA and MAOB genes in mental disorders. J Neural Transm. 125:1581–1588. DOI:10.1007/s00702-018-1929-6.