Phylogeography of the Maleo Senkawor (Macrocephalon maleo Sal. Muller 1846) Based on Cytochrome B Gene in Sulawesi And Their Sex Determination using Molecular Sexing

Abdul Samad, D D Solihin, D D Solihin, Cece Sumantri, Cece Sumantri, B Purwantara, B Purwantara
 DOI  https://doi.org/10.14334/jitv.v28i1.3107

Abstract

The Maleo Senkawor bird (Macrocephalon maleo) is endemic and widely distributed in Sulawesi. Maleo Senkawor bird is monomorphic so it is difficult to distinguish between male and female individuals. This study aimed to determine the kinship relationship among M. maleo in various geographic areas in Sulawesi through the use of the gene marker Cytochrome-b (Cyt-b), determine the sex ratio, and examine the reliability of the 2550F/2718R primer. Fifteen (feather and eggshell) samples from North Sulawesi, Southeast Sulawesi, and Central Sulawesi were collected. Total DNA isolation was performed using the Dneasy® Blood and Tissue kit (Cat. No. 69504) following a Qiagen protocol with modification. PCR amplification (35 cycles) used a forward primer MMCytb_F (5'- GAAAATCCCACCCCCTACTA-3'), a reverse primer MMCytb_R (5'- GTTGGCTACGAGGAGTCAGA-3') and a primer for sexing used 2550F/2718R. Analysis was performed on the length of 903 bp Cyt-b mtDNA gene sequences of M. maleo along with their amino acid sequences (301 AA) and Chromo Helicase DNA-Binding (CHD) gene sequences. Based on the genetic distance of the Kimura 2-Parameter model and p-distance, the interpopulation of M. maleo in Sulawesi (the population of Southeast Sulawesi was separated from the population of Central Sulawesi and North Sulawesi) was 0.002 (0.2%) – 0.003 (0.3%). Each population has formed a different haplotype. Primer 2550F/2718R was able to amplify the CHD gene and could distinguish the sex identification. Male individuals are more dominant than female individuals with a sex ratio of 6,5:1

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Amiri N, Vaissi S, Aghamir F, Saberi-Pirooz R, Rödder D, Ebrahimi E, Ahmadzadeh F. 2021. Tracking climate change in the spatial distribution pattern and the phylogeographic structure of Hyrcanian wood frog, Rana pseudodalmatina (Anura: Ranidae). J Zool Syst Evol Res. 59:1604–1619. DOI:10.1111/jzs.12503.

Anucherngchai S, Chontananarth T, Tejangkura T, Chai JY. 2019. The study of Cytochrome B (CYTB): species-specific detection and phylogenetic relationship of Echinostoma revolutum, (Froelich, 1802). J Parasit Dis. 43:66–74. DOI:10.1007/s12639-018-1057-0.

Arista K, Wahid A, Ihsan M. 2015. Faktor penyebab penurunan populasi Maleo Senkawor di Desa Sausu Piore, Kabupaten Parigi Moutong, Sulawesi Tengah. War Rimba. 3:1–8.

Awad A, Khalil, Abd-Elhakim. 2015. Molecular phylogeny of some avian species using Cytochrome b gene sequence analysis.

Bandelt H-J, Forster P, Röhl A. 1999. Median-Joining Networks for Inferring Intraspecific Phylogenies. Mol Biol Evol. 16:37–48.

Begović L, Mihić I, Pospihalj T, Mikuška T, Mlinarić S, Mikuška A. 2017. Evaluation of methods for molecular sex-typing of three heron species from different DNA sources. Turkish J Zool. 41:593–598. DOI:10.3906/zoo-1602-47.

Çakmak E, Akın Pekşen Ç, Bilgin CC. 2017. Comparison of three different primer sets for sexing birds. J Vet Diagnostic Investig. 29:59–63. DOI:10.1177/10406387 16675197.

Çoraman E, Furman A, Karataş A, Bilgin R. 2013. Phylogeographic analysis of Anatolian bats highlights the importance of the region for preserving the Chiropteran mitochondrial genetic diversity in the Western Palaearctic. Conserv Genet. 14:1205–1216. DOI:10.1007/s10592-013-0509-4.

Demarchi B, Stiller J, Grealy A, Mackie M, Deng Y, Gilbert T, Clarke J, Legendre LJ, Boano R, Sicheritz-Ponten T, Magee J, Zhang G, Bunce M, Collins MJ, Miller G. 2022. Ancient proteins resolve controversy over the identity of Genyornis eggshell. PNAS. e2109326119. DOI:10.1073/pnas.2109326119 j.

Drahulian M, Chaplygina A, Savynska N, Kostenko S, Ostrovskyi P, Gusar K. 2018. The physiological and genetic differences between flycatchers (Ficedula albicollis vs. Ficedula hypoleuca). Folia Oecologica. 45:111–119.DOI:10.2478/foecol-2018-0012

Fridolfsson A-K, Ellegren H. 1999. Short Communications. J AVIAN Biol. 30:116–121. DOI:10.1163/003925995X0 0233.

Froese GZL, Mustari AH. 2019. Assessments of Maleo Macrocephalon maleo nesting grounds in South-east Sulawesi reveal severely threatened populations. Bird Conserv Int. 29:497–502. DOI:10.1017/S0959270918 000333

Fünfstück T, Vigilant L. 2015. The geographic distribution of genetic diversity within gorillas. Am J Primatol. 77:974–985. DOI:10.1002/ajp.22427.

Hall R. 2001. Cenozoic reconstructions of SE Asia and the SW Pacific: Changing patterns of land and sea. Faunal Flor Migr Evol SE Asia-Australasia.(Figure 1):35–56.

Harris RB, Birks SM, Leaché AD. 2014. Incubator birds: Biogeographical origins and evolution of underground nesting in megapodes (Galliformes: Megapodiidae). J Biogeogr. 41:2045–2056. DOI:10.1111/jbi.12357.

Harrisson KA, Magrath MJL, Yen JDL, Pavlova A, Murray N, Quin B, Menkhorst P, Miller KA, Cartwright K, Sunnucks P. 2019. Lifetime Fitness Costs of Inbreeding and Being Inbred in a Critically Endangered Bird. Curr Biol 29 (16): 2711-2717. DOI:10.1016/j.cub.2019.06.064.

Hedrick PW, Garcia-Dorado A. 2016. Understanding Inbreeding Depression, Purging, and Genetic Rescue. Trends Ecol Evol. 31:940–952. DOI:10.1016/J.TREE .2016.09.005.

Ibis O, Tez C, Özcan S. 2014. Phylogenetic Status of the Turkish Red Fox (Vulpes vulpes), based on Partial Sequences of the Mitochondrial Cytochrome-b Gene. Vertebrate Zoology. 64: 273 – 284.

Işçi B, Yildirim HK, Altindisli A. 2014. Evaluation of methods for DNA extraction from must and wine. Journal of the Institute of Brewing. 120(3): 238-243. DOI:10.1002/jib.129.

Kaunisto KM, Roslin T, Sääksjärvi IE, Vesterinen EJ. 2017. Pellets of proof: First glimpse of the dietary composition of adult odonates as revealed by metabarcoding of feces. Ecol Evol. 7:8588–8598. DOI:10.1002/ece3.3404.

Kato H, Gomez EA, Martini-Robles L, Muzzio J, Veles L, Calpovina M, Romero-Alvares D, Mimori T, Uezato H, Hashiguchi Y. 2016. Geographic distribution of Leishamina species in Ecuador based on the Cytochrome B gene sequence analisys. PLoS Negl Trop Dis 10: e0004844. DOI:10.1371/journal.pntd.0004844.

Khaerunnisa I, Sari E, Ulfah M, Jakaria, Sumantri C. 2013. Avian sex determination based on chromo helicase DNA-binding (CHD) genes using polymerase chain reaction (PCR). Media Peternak. 36:85–90. DOI:10.5398/medpet.2013.36.2.85.

Kumar S, Stecher G, Tamura K, Dudley J. 2016. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Mol Biol Evol. 33:1870–1874. DOI:10.1093/molbev/msw054.

Kus BE, Howell SL, Wood DA. 2017. Female-biased sex ratio, polygyny, and persistence in the endangered Southwestern Willow Flycatcher (Empidonax traillii extimus). 119:17–25. DOI:10.1650/CONDOR-16-119.1.

Lapinski AG, Pavlenko MV, Solovenchuk LL, Gorbachev V V. 2016. Some limitations in the use of the mitochondrial DNA cytb gene as a molecular marker for phylogenetic and population-genetic studies by the example of the Apodemus genus. Russ J Genet Appl Res. 6:84–90. DOI:10.1134/S2079059716010093.

Maia TA, Vilaça ST, da Silva LR, Santos FR, Dantas GPDM. 2017. DNA sampling from eggshells and microsatellite genotyping in rare tropical birds: Case study on Brazilian Merganser. Genetics and Molecular Biology. 40: 808-812. DOI:10.1590/1678-4685-GMB-2016-0297.

Osman MA, Sugnaseelan S, Jothi |, Panandam M, Putra Malaysia U, Correspondence M, Izza N, Ghani A. 2020. Molecular sex identification of Malaysian White-Nest Swiftlet (Aerodramus fuciphagus Thunberg, 1812) [Internet]. https://doi.org/10.1002/ece3.6699

Pekkala N, Knott KE, Kotiaho JS, Nissinen K, Puurtinen M. 2014. The effect of inbreeding rate on fitness, inbreeding depression and heterosis over a range of inbreeding coefficients. 7(9): 1107-1119. DOI:10.1111/eva.12145.

Peters C, Nelson · Howard, Rusk B, Muir A. 2020. A novel method to optimise the utility of underused moulted plumulaceous feather samples for genetic analysis in bird conservation. 12:457–467. DOI:10.1007/s12686-019-01117-8.

Petrova TV, Zakharov ES, Samiya R, Abramson NI. 2014. Phylogeography of the narrow-headed vole Lasiopodomys (Stenocranius) gregalis (Cricetidae, Rodentia) inferred from mitochondrial cytochrome b sequences: an echo of Pleistocene prosperity. J Zoolog Syst Evol Res. 53(2): 97-108. DOI: 10.1111/jzs.12082.

Purwaningrum M, Nugroho HA, Asvan M, Karyanti K, Alviyanto B, Kusuma R, Haryanto A. 2019. Research Centre for Biology, Indonesian Institute of Sciences. Gembira Loka Zoo Bot Gard. 2. DOI:10.14202/ vetworld.2019.1506-1513.

Ravindran S, Woo WK, Saufi S, Amni WN, Hamid NH, Abidin CMRZ, Ishak I, Azzam G, Salim H. 2019. Molecular sexing of southeast asian barn owl, tyto alba javanica, using blood and feather. Trop Life Sci Res. 30:13–23. DOI:10.21315/tlsr2019.30.2.2.

Rozas J, Ferrer-Mata A, Carlos anchez-DelBarrio JS, Guirao-Rico S, Librado P, an Ramos-Onsins SE, anchez-Gracia AS. 2017. DnaSP 6: DNA Sequence Polymorphism Analysis of Large Data Sets. DOI:10.1093/mol bev/msx248.

Rytkönen S, Vesterinen EJ, Westerduin C, Leviäkangas T, Vatka E, Mutanen M, Välimäki P, Hukkanen M, Suokas M, Orell M. 2019. From feces to data: A metabarcoding method for analyzing consumed and available prey in a bird-insect food web. Ecol Evol. 9:631–639. DOI:10.1002/ece3.4787.

Shekelle M, Leksono SM. 2019. Strategi konservasi di Pulau Sulawesi dengan menggunakan tarsius sebagai flagship spesies. Biota J Ilm Ilmu-Ilmu Hayati. 9 (1): 1-10. DOI:10.24002/biota.v9i1.2791.

Spiridonova LN, Val’chuk OP, Belov PS, Maslovsky KS. 2013. Intraspecific genetic differentiation of the Siberian Rubythroat (Luscinia calliope): Data of sequencing the mtDNA cytochrome b gene. Russ J Genet. 49:638–644. DOI:10.1134/S1022795413060136.

Szekely T, Weissing FJ, Komdeur J. 2014. Adult sex ratio variation: implications for breeding system evolution. J Evol Biol. 27:1500–1512. DOI:10.1111/jeb.12415.

Vucicevic M, Stevanov-Pavlovic M, Stevanovic J, Bosnjak J, Gajic B, Aleksic N, Stanimirovic Z. 2013. Sex determination in 58 bird species and evaluation of CHD Gene as a universal molecular marker in bird sexing. Zoo Biol. 32:269–276. DOI:10.1002/zoo.21010.

Widnyanya I. 2017. Kajian morfologi dan morfometri telur burung Maleo (Macrocephalon maleo SAL. Muller 1846). Agropet. 14(1). ISSN:1693-9158.

Yalçınkaya B, Yumbul E, Mozioğlu E, Akgoz M. 2017. Comparison of DNA extraction methods for meat analysis. Food Chem. 221:1253–1257. DOI:10.1016/J. FOODCHEM.2016.11.032.

Yong H Sen, Eamsobhana P, Song SL, Prasartvit A, Lim PE. 2015. Molecular phylogeography of Angiostrongylus cantonensis (Nematoda: Angiostrongylidae) and genetic relationships with congeners using cytochrome b gene marker. Acta Trop. 148:66–71. DOI:10.1016/J.ACTA TROPICA.2015.04.020.

Yuda P, Saputra AW. 2021. Eggshell membrane for DNA sexing of the endangered Maleo (Macrocephalon maleo). Version 4 F1000Research. 9:599. DOI:10.12688/f1000research.23712.1.

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