Assessment of sperm acrosome status, malondialdehyde and aspartate aminotransferase enzyme concentration of frozen semen from Limousin and Simmental bulls in different commercial diluents

Muhammad Agus Tahar, Komariah Komariah, Henny Nuraini, Tulus Maulana, Muhammad Gunawan, R Iis Arifiantini
 DOI  https://doi.org/10.14334/jitv.v27i3.3049

Abstract

Sperm cryopreservation is the process of preserving sperm cells at low temperatures, so that its frozen semen can be used in the future. The quality of the frozen sperm is affected by the diluent. The objective of this study was to compare the effects of commercial diluents on acrosome status, malondialdehyde (MDA) and aspartate aminotransferase (AspAT) enzyme concentration of thawed Limousin and Simmental bull semen. Semen was collected twice weekly using an artificial vagina. The fresh semen processed into frozen semen had  sperm motility of >70%. The one-step procedure was used for the dilution methods. Andromed®, Optixcell® and Steridyl® were used as diluents. Data were analyzed by analysis of variance (ANOVA) followed by Tukey HSD 5% confidence interval. The result showed no interaction (P>0.05) between two factors on acrosome status. The sperm acrosome damage of Simmental in Steridyl® was significantly lower than others (P<0.05), although all diluents showed low sperm acrosome damage. Also, no interaction between the type of diluent and breed on MDA and AspAT enzyme concentrations was detected (P>0.05). The results suggest that three commercial diluents have equal efficacy in protecting acrosome status and maintaining MDA and AspAT enzyme concentrations of frozen Limousin and Simmental bull semen. Therefore, all commercial diluents can be an alternative for Limousin and Simmental frozen semen.

Keywords

Acrosome Status; AspAT; MDA; Limousin and Simmental Bull

Full Text:

PDF

References

[BSN] Badan Standarisasi Nasional. 2017. Semen Beku Bagian 1:Sapi. Jakarta (Indones): Badan Standarisasi Nasional.

Ahmadi S, Bashiri R, Ghadiri-Anari A, Nadjarzadeh A. 2016. Antioxidant supplements and semen parameters: An evidence based review. Int J Reprod Biomed. 14:729–736. DOI:10.29252/ijrm.14.12.729.

Arif AA, Maulana T, Kaiin EM, Purwantara B, Arifiantini RI, Memili E. 2020. Comparative analysis of various step-dilution techniques on the quality of frozen Limousin bull semen. Vet World. 13:2422–2428. DOI:10.14202/ vetworld.2020.2422-2428.

Arifiantini R. 2012. Technique of semen collection and evaluation in animals. Bogor (Indones): IPB Press.

Asadpour R, Taravat M, Rahbar M, Khoshniyat M, Hamidian G. 2021. Effects of vitamin D supplementation in extender on sperm kinematics and apoptosis following the freeze-thaw process in normozoospermic and asthenozoospermic Holstein bulls. Basic Clin Androl. 31:20. DOI:10.1186/s12610-021-00137-5.

Dwinofanto H, Rimayanti R, Mustofa E, Susilowati S, Hernawati T. 2019. The effect of duration of preservation on the quality, MDA level, and DNA damage of post-thawed Bali cattle bull sperm. Iraqi J Vet Sci. 32:249–252. DOI:10.33899/ijvs.2019.153857.

El-Sharawy M, Eid E, Darwish S, Abdel-Razek I, Islam MR, Kubota K, Yamauchi N, El-Shamaa I. 2017. Effect of organic and inorganic selenium supplementation on semen quality and blood enzymes in buffalo bulls. Anim Sci J. 88:999–1005. DOI:10.1111/asj.12736.

Fraser L, Dziekońska A, Strzeżek R, Strzeżek J. 2007. Dialysis of boar semen prior to freezing–thawing: Its effects on post-thaw sperm characteristics. Theriogenology. 67:994–1003. DOI:10.1016/j.therioge nology.2006.12.002.

Fraser L, Zasiadczyk Å, Pareek CS. 2018. Effects of boar variability on comet-detected sperm-DNA damage following cryopreservation. Anim Prod Sci. 58:252. DOI:10.1071/AN16274.

Frydrychová S, Čeřovský J, Lustyková A, Rozkot M. 2010. Effects of long-term liquid commercial semen extender and storage time on the membrane quality of boar semen. Czech J Anim Sci. 55:160–166. DOI:10. 17221/62/2009-CJAS.

Gączarzewicz D, Piasecka M, Udała J, Błaszczyk B, Stankiewicz T, Laszczyńska M. 2010. Plasma membrane changes during the liquid storage of boar spermatozoa: A comparison of methods. Acta Vet Hung. 58:105–116. DOI:10.1556/avet.58.2010.1.11.

Hammad M, Wafa W, Gabr A, Elkishky A. 2019. Different types and levels of Moringa oleifera leaf extract as a source of antibiotics in Friesian bull semen extender. J Anim Poult Prod. 10:67–71. DOI:10.21608/japp mu.2019.40526.

Hezavehei M, Sharafi M, Kouchesfahani HM, Henkel R, Agarwal A, Esmaeili V, Shahverdi A. 2018. Sperm cryopreservation: A review on current molecular cryobiology and advanced approaches. Reprod Biomed Online. 37:327–339. DOI:10.1016/j.rbmo.2018.05.012.

Hidayati B, Arifiantini R, Karja N, Kusumaningrum D. 2018. Kualitas semen kambing Sapera yang dibekukandalam pengencer Tris kuning telur dengan imbuhan pentoxifylline. J Vet. 19:404–411. DOI:10.19087/jvet eriner.2018.19.404.

Indriastuti R, Ulum MF, Arifiantini RI, Purwantara B. 2020. Individual variation in fresh and frozen semen of Bali bulls (Bos sondaicus). Vet World. 13:840–846. DOI: 10.14202/vetworld.2020.840-846.

Isnaini N, Ihsan MN, Wahjuningsih S. 2019. Mangosteen peel extract in tris-egg yolk extender improves fertility of cryopreserved goat sperm. LRRD. 31.

Khumran AM, Yimer N, Rosnina Y, Ariff MO, Wahid H, Kaka A, Ebrahimi M, Sarsaifi K. 2015. Butylated hydroxytoluene can reduce oxidative stress and improve quality of frozen–thawed bull semen processed in lecithin and egg yolk based extenders. Anim Reprod Sci. 163:128–134. DOI:10.1016/j.anireprosci.2015.10.007.

Lopes SAF, Rosa HJD, Chaveiro A, da Silva FM. 2021. Influence of different freezing curves on the acrosome integrity of male goat sperm cells. Am J Anim Vet Sci. 16:56–61. DOI:10.3844/ajavsp.2021.56.61.

Morrell J, Wallgren M. 2014. Alternatives to antibiotics in semen extenders: a review. Pathogens. 3:934–946. DOI:10.3390/pathogens3040934.

Nguyen V V, Ponchunchoovong S, Kupittayanant S, Kupittayanant P. 2019. Effects of egg yolk and soybean lecithin on sperm quality determined by computerâ€assisted sperm analysis and confocal laser scanning microscope in chilled canine sperm. Vet Med Sci. 5:345–360. DOI:10.1002/vms3.158.

Nofa Y, Karja NWK, Arifiantini RI. 2018. Acrosome status and quality of post-thawed sperm from several cattle breed of two artificial insemination centre. Acta Veterninaria Indones. 5:81–88. DOI:10.29244/avi.5.2. 81-88.

du Plessis S, Agarwal A, Mohanty G, van der Linde M. 2015. Oxidative phosphorylation versus glycolysis: what fuel do spermatozoa use? Asian J Androl. 17:230. DOI:10.4 103/1008-682x.135123.

Prihantoko KD, Yuliastuti F, Haniarti H, Kusumawati A, Widayati DT, Budiyanto A. 2020. The acrosome integrity examination of post-thawed spermatozoa of several Ongole grade bull in Indonesia using Giemsa staining method. IOP Conf Ser Earth Environ Sci. 478:012042. DOI:0.1088/1755-1315/478/1/012042.

Rajabi-Toustani R, Akter QS, Almadaly EA, Hoshino Y, Adachi H, Mukoujima K, Murase T. 2019. Methodological improvement of fluorescein isothiocyanate peanut agglutinin (FITC-PNA) acrosomal integrity staining for frozen-thawed Japanese Black bull spermatozoa. J Vet Med Sci. 81:694–702. DOI:10.1292/jvms.18-0560.

Rizal M, Riyadhi M. 2016. Fertilitas semen kerbau Rawa (Bubalus bubalis carabanensis) yang diencerkan dengan pengencer nira aren. J Vet. 17:457–467. DOI:10.19087 /jveteriner.2016.17.3.457.

Sharafi M, Zhandi M, Shahverdi A, Shakeri M. 2015. Beneficial effects of nitric oxide induced mild oxidative stress on post-thawed bull semen quality. Int J Fertil Steril. 9:230–237.

Subramanian V, Ravichandran A, Thiagarajan N, Govindarajan M, Dhandayuthapani S, Suresh S. 2018. Seminal reactive oxygen species and total antioxidant capacity: Correlations with sperm parameters and impact on male infertility. Clin Exp Reprod Med. 45:88–93. DOI:10.5653/cerm.2018.45.2.88.

Soeparna, Arifiantini RI. 2013. Fisiologi Reproduksi dan Inseminasi Buatan pada Kuda. Bogor (Indones): IPB Press.

Sukmawati E, Arifiantini RI, Purwantara B. 2015. Freezing capacity of sperm on various type of superior bulls. J Ilmu Ternak dan Vet. 19:168–175. DOI:10.14334 /jitv.v19i3.1079.

Susilawati T. 2013. Pedoman inseminasi buatan pada ternak. Malang (Indones): UB Press.

Tejaswi V, Swamy MN, Yathiraj S, Honnappa TG, Isloor S. 2016. Enzymatic activities in fresh seminal plasma and extended refrigerated semen in Nari suvarna rams. Theriogenology Insight-An Int J Reprod all Anim. 6(1):27. DOI:10.5958/2277-3371.2016.00003.6.

Tsikas D. 2017. Assessment of lipid peroxidation by measuring malondialdehyde (MDA) and relatives in biological samples: Analytical and biological challenges. Anal Biochem. 524:13–30. DOI:10.1016 /j.ab.2016.10.021.

Ugur MR, Saber Abdelrahman A, Evans HC, Gilmore AA, Hitit M, Arifiantini RI, Purwantara B, Kaya A, Memili E. 2019. Advances in cryopreservation of bull sperm. Front Vet Sci. 6:1–15. DOI:10.3389/fvets.2019.00268.

Üstüner B, Nur Z, Alçay S, Toker MB, Sağirkaya H, Soylu MK. 2015. Effect of freezing rate on goat sperm morphology and DNA integrity. Turkish J Vet Anim Sci. 39:110–114. DOI:10.3906/vet-1407-70.

Zaenuri LA, Lukman L, Yanuarianto O, Sumadiasa IWL, Rodiah R. 2018. Additional freeze drying Fig Fruit (Ficus carica L) filtrate into tris egg yolk extender and its effect on sperm membrane integrity and acrosome of Kacang buck. Anim Prod. 19:161. DOI:10.20884 /1.jap.2017.19.3.647.

Zamuna K, Susilawati T, Ciptadi G, Marjuki M. 2015. Differences semen quality and frozen semen production in various breeds of beef cattle. JTAPRO. 16:1–6. DOI: 10.21776/ub.jtapro.2015.016.02.1.

Refbacks

  • There are currently no refbacks.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.



Bookmark and Share