Correlation between Semen Quality, Libido, and Testosterone Concentration in Bali Bulls

Hikmayani Iskandar, Herry Sonjaya, Raden IIS Arifiantini, Hasbi Hasbi

Abstract

Indonesia has two National Artificial Insemination Centers (AIC) and more than 15 Regional Artificial Insemination Centers (RAIC) spread across several provinces. Bulls in the AIC must have a high libido and produce good quality semen. This study examines the correlation between libido with semen quality and testosterone concentration to determine potential frozen semen production from Bali bulls in South Sulawesi RAIC. Ten Bali bull were used in this study. Semen collection was carried out twice a week with semen evaluation following the RAIC protocol. At the same time, blood samples and libido measurements were carried out from each bull. The frozen semen production potential was calculated by multiplying the semen volume, motility, and sperm concentration. The results showed that the quality of fresh semen and testosterone concentrations did not differ between high and low libido of Bali bulls. Libido has a positive correlation with semen volume (r= 0.52) and sperm motility (r= 0.62), while testosterone concentration has a negative correlation with semen volume (r= -0.65), sperm motility (r= -0.60), and libido (r= -0.48). Bulls with high and low libido have good frozen semen production potential, ranging from 19,755 – 21,640 straws per year. Bali bulls in RAIC have fresh semen quality and testosterone concentrations under normal conditions, with high potential for frozen semen production, although only 60% of Bali cattle have high libido and 40% have low libido.

Keywords

Bali Bull; Frozen Semen Productivity; Libido; Semen Quality; Testosterone Concentrationull, libido, frozen semen productivity, semen quality, testosterone concentration

Full Text:

PDF

References

[BSN] Badan Standarisasi Nasional. 2017. SNI Semen Beku-Bagian 1: Sapi. Jakarta (Indones): Badan Standardisasi Nasional.

[Ditjennak] Direktorat Perbibitan dan Produksi Ternak. 2018. Roadmap swasembada pejantan unggul 2018-2022. Jakarta (Indones): Direktorat Perbibitan dan Produksi Ternak.

Abell KM, Theurer ME, Larson RL, White BJ, Hardin DK, Randle RF, Cushman RA. 2017. Calving distributions of individual bulls in multiple-sire pastures. Theriogenology. 93:7–11. DOI: 10.1016/j.theriogenolo gy.2017.01.010.

Alwiyah A, Naraini H, Agung P, Jakaria J. 2016. Polymorphism stearoyl-CoA desaturase (SCD) gene and association with characteristics meat in Bali cattle. J Indones Trop Anim Agric. 41:188. DOI: 10.14710/jitaa. 41.4.188-195.

Andersson M. 1992. Relationships between GnRH-induced testosterone maxima, sperm motility and fertility in Ayrshire bulls. Anim Reprod Sci. 27:107–111. DOI:L 10.1016/0378-4320(92)90050-N.

Ansari MS, Ali S, Ullah N, Anwar M. 2007. Blood serum testosterone level and its relationship with scrotal circumference and semen characteristics in Nili-Ravi Buffalo bulls. Pak Vet J. 27:63–66.

Anwar P, Jiyanto J. 2019. Identifikasi hormon testosteron sapi Kuantan plasma nutfah Riau sebagai penentu klasifikasi kriteria pejantan unggul. J Peternak Indones. 21:230. DOI: 10.25077/jpi.21.3.230-239.2019.

Arifiantini R. 2012. Semen collection and evaluation techniques in animals. Bogor (Indones): IPB Press.

Ax R, Dally M, Didion B, Lenz R, Love C, Varner D, Hafez B, Bellin M. 2000. Artificial insemination. In: Hafez B, Hafez E, editors. Reprod Farm Anim. 7th ed. Philadelphia (US): Wiley-Blackwell.

Baharun A, Said S, Arifiantini RI, Karja NWK. 2021. Correlation between age, testosterone and adiponectin concentrations, and sperm abnormalities in Simmental bulls. Vet World. 14:2124–2130. DOI: 10.14202/ vetworld.2021.2124-2130

Chacur M, Arikawa A, Oba E, Souza C, Roberto Gabriel Filho L. 2018. Influence of testosterone on body and testicular development in Zebu cattle in the tropical climate. In: Estrada M, editor. Adv Testosterone Action. London (UK): IntechOpen; p. 18.

Chacur MGM, Mizusaki KT, Filho LRAG, Oba E, Ramos AA. 2013. Seasonal effects on semen and testosterone in zebu and taurine bulls. Acta Sci Vet. 41.

Chenoweth P. 2021. Bull behavior, sex-drive and management. In: Fields M, Sand R, editors. Factors Affect Calf Crop. Boca Raton: CRC Press; p. 319–330. DOI:10.1201/97810030691 19-23.

Le Danvic C, Gérard O, Sellem E, Ponsart C, Chemineau P, Humblot P, Nagnan-Le Meillour P. 2015. Enhancing bull sexual behavior using estrus-specific molecules identified in cow urine. Theriogenology. 83:1381–1388. DOI: 10.1016/j.theriogenology.2015.02.004.

Dasrul D, Wahyuni S, Sugito S, Hamzah A, Zaini Z, Haris A, Gholib Gholib. 2020. Correlation between testosterone concentrations with scrotal circumference, and semen characteristics in Aceh bulls.Gholib G., Sutriana A, Engelhardt A, Duboscq J, Sahara Zamzami R, editors. E3S Web Conf. 151:01015. DOI:10.1051/e3sconf/2020 15101015.

Garner DL, Hafez ESE. 2016. Spermatozoa and Seminal Plasma. In: Reprod Farm Anim. Baltimore, Maryland, USA: Lippincott Williams & Wilkins; p. 96–109. DOI:10.1002/9781119265306.ch7.

Gholami H, Towhidi A, Zare Shahneh A, Dirandeh E. 2010. The relationship of plasma leptin concentration and puberty in Holstein bull calves (Bos taurus). J Anim Physiol Anim Nutr (Berl). 94:797–802. DOI:10.1111 /j.1439-0396.2009.00970.x

Gholib G, Wahyuni S, Akmal M, Hasan M, Agil M, Purwantara B. 2020. The validation of a commercial enzyme-linked immunosorbent assay and the effect of freeze-thaw cycles of serum on the stability of cortisol and testosterone concentrations in Aceh cattle. F1000Research. 8:1220. DOI: 10.12688/f1000research. 19804.3.

Gulia S, Sarkar M, Kumar V, Meyer H, Prakash B. 2010. Divergent development of testosterone secretion in male zebu (Bos indicus) and crossbred cattle (Bos indicus x Bos taurus) and buffaloes (Bubalus bubalis) during growth. Trop Anim Health Prod. 42:1143–1148. DOI:10.1007/s11250-010-9538-x.

Gunawan I, Suwiti N, Sampurna P. 2016. The effects of mineral on the chest circumference, body length, and body height of male Bali cattle. Bul Vet Udayana. 8:128–134.

Hafizuddin H, Karja NWK, Praharani L, Setiadi MA. 2020. Adiponectin and testosterone level and their correlations with fertility in Anglo Nubian×Etawah grade crossbred bucks. Trop Anim Sci J. 43:110–116. DOI:10.5398/ tasj.2020.43.2.110.

Hapsari RD, Khalifah Y, Widyas N, Pramono A, Prastowo S. 2018. Age effect on post freezing sperm viability of Bali cattle (Bos javanicus). IOP Conf Ser Earth Environ Sci. 142:012007. DOI:10.1088/1755-1315/142/1/012007.

Hastono H, Praharani L. 2014. Evaluation of libido and mating ability of bulls from Friesian Holstein and Ongole crossbreed. In: Pros Semin Nas. Bogor (Indones): Lembaga Ilmu Pengetahuan Indonesia.

He Z-X, Huang M-F, Wang A-K, Zhang J-C, Zhao G, Yuan X-P, Qu K-X, Huang B-Z. 2014. Patterns of plasma hormone concentrations in Mithun bulls under a semi-management. J Anim Vet Adv. 13:732–739.

Hoflack G, Van Soom A, Maes D, de Kruif A, Opsomer G, Duchateau L. 2006. Breeding soundness and libido examination of Belgian Blue and Holstein Friesian artificial insemination bulls in Belgium and The Netherlands. Theriogenology. 66:207–216. DOI:10. 1016/j.theriogenology.2005.11.003.

Indrayanto Y. 2021. Andropause. Surakarta (Indones): Sebelas Maret University.

Indriastuti R, Ulum MF, Arifiantini RI, Purwantara B. 2020. Individual variation in fresh and frozen semen of Bali bulls (Bos sondaicus). Vet World. 13:840–846. DOI:10. 14202/vetworld.2020.840-846

Islam M, Apu A, Hoque S, Ali M, Karmaker S. 2018. Comparative study on the libido, semen quality and fertility of Brahman cross, Holstein Friesian cross and Red Chittagong breeding bulls. Bangladesh J Anim Sci. 47:61–67. DOI: 10.3329/bjas.v47i2.40236.

Ismaya. 2014. Biotechnology of artificial insemination on cattle and buffalo. Yogyakarta (Indones): UGM Press.

Jakaria, Khasanah H, Priyanto R, Baihaqi M, Ulum MF. 2017. Prediction of meat quality in Bali cattle using ultrasound imaging. J Indones Trop Anim Agric. 42:59. DOI: 10.14710/jitaa.42.2.59-65

Kowalczyk A, Gałęska E, Czerniawska-Piątkowska E, Szul A, Hebda L. 2021. The impact of regular sperm donation on bulls’ seminal plasma hormonal profile and phantom response. Sci Rep. 11(1):11116. DOI: 10.1038/s41598-021-90630-8.

Littlejohn BP, Roberts MC, Bedenbaugh MN, Lewis AW, Neuendorff DA, Riley DG, Carroll JA, Vann RC, Amstalden M, Randel RD, Welsh TH. 2017. Evaluation of the influence of prenatal transportation stress on GnRH-stimulated luteinising hormone and testosterone secretion in sexually mature Brahman bulls1,2. J Anim Sci. 95:129–138. DOI: 10.2527/jas.2016.0445.

Mahmood S, Ahmad N, Rehman H, Zaneb H, Farooq U. 2014. A study on relationship among age, body weight, orchidometry and semen quality parameters in adult cholistani breeding bulls. J Anim Plant Sci. 2425:380–384.

Malik M, Jamil H, Qureshi Z, Mehfooz A, Rizvi S, Ullah S, Dilshad S, Zaman A, Ullah N, Safdar S, et al. 2018. Investigation on relationship of hormonal profile and biochemical constituents of seminal plasma with physical characteristics of Damani buck semen. Pure Appl Biol. 7:684–691.

Manegassi S, Peripolli V, Canozzi M, Barcellos J. 2021. Libido and serving skills of Zebu cattle bulls. Semina. 42:3057–3063.

Masoumi R, Towhidi A, Javaremi A, Nabizadeh H, Zhandi M. 2011. Influence of PGF2α on semen quality and libido in Holstein bulls. Turkish J Vet Anim Sci. 35:1–6.

Minister of Agriculture of Republic of Indonesia. 2016. Peraturan Menteri Pertanian Republik Indonesia. Jakarta (Indones), Indonesia.

Mohammed A, Ahmed W. 2017. Pure Egyptian cattle bulls show both individual variation and different interaction with extender in the post-thawing sperm parameters. Andrology. 6:10000193.

Morrell JM, Valeanu AS, Lundeheim N, Johannisson A. 2018. Sperm quality in frozen beef and dairy bull semen. Acta Vet Scand. 60:41. DOI:10.1186/s13028-018-0396-2.

Mukhopadhyay CS, Gupta AK, Yadav BR, Khate K, Raina VS, Mohanty TK, Dubey PP. 2010. Subfertility in males: An important cause of bull disposal in bovines. Asian-Australasian J Anim Sci. 23:450–455. DOI:10.5713/ajas.2010.90298.

Murphy C, Fahey AG, Shafat A, Fair S. 2013. Reducing sperm concentration is critical to limiting the oxidative stress challenge in liquid bull semen. J Dairy Sci. 96:4447–4454. DOI:10.3168/jds.2012-6484.

Nugraha CD, Widodo N, Kuswati K, Suyadi S. 2022. The real potential of semen production of Bali Bull: over year observation at Singosari National Artificial Insemination Center (SNAIC), Singosari-Indonesia.Widodo E, Jayanegara A, Suyadi, Nurgiartiningsih VMA, Ciptadi G, Natsir MH, Wahjuningsih S, Sjofjan O, Marjuki, editors. E3S Web Conf. 335:00045. DOI:10.1051/e3sconf /202233500045.

Perumal P, Chang S, De AK, Baruah KK, Khate K, Vupru K, Mitra A. 2020. Slow release exogenous melatonin modulates scrotal circumference and testicular parameters, libido, endocrinological profiles and antioxidant and oxidative stress profiles in mithun. Theriogenology. 154:1–10.

Rachmawati L, Ismaya, Astuti P. 2014. Correlation between testosterone, libido and sperm quality of Bligon, Kejobong and Ottawa grade bucks. Bul Peternak. 38:8. DOI:10.21059/buletinpeternak.v38i1.4598.

Rajak S, Tripathi U, Attupuram N, Boro P, Layek S, Aslam M, Kumaresan A, Mohanty T, Sreela L, Prakash M. 2014a. Relationship of blood and seminal plasma testosterone concentrations with semen quality in crossbred bulls. Indian J Dairy Sci. 67:162–167.

Rajak SK, Kumaresan A, Gaurav MK, Layek SS, Mohanty TK, Muhammad Aslam MK, Tripathi UK, Prasad S, De S. 2014b. Testicular cell indices and peripheral blood testosterone concentrations in relation to age and semen quality in crossbred (Holstein Friesian×Tharparkar) bulls. Asian-Australasian J Anim Sci. 27:1554–1561. DOI:10.5713/ajas.2014.14139.

Sankhi S, Sapkota KR, Regmi B. 2019. Effect of age and frequency of collection on quality of Jersey bulls semen at National Livestock Breeding Center (NLBC), Nepal. Int J Appl Sci Biotechnol. 7:88–95. DOI: 10.3126 /ijasbt.v7i1.23312.

Santos MD, Torres CAA, Ruas JRM, Guimarães JD, Silva Filho JM. 2004. Reproductive potential of Nelore bulls submitted to different bull:cow proportion. Arq Bras Med Vet e Zootec. 56:497–503.

Schallenberger E, Hartl P, Schams D, Lorrmann W, Hahn R. 1991. Hormone profiles and fertility in bulls. Tierzuchter 43:402-403. Tierzuchter. 43:402–403.

Sekasiddhi P, Buban S. 1997. Hormone profiles in low libido bulls. J Thai Vet Med Asso. 48:43–49.

Senger P. 2012. Pathways to pregnancy and parturition. 3rd ed. Redmond (USA): Current Conceptions. Inc.

Singh AK, Brar PS, Cheema RS. 2014. Relationships among frozen-thawed semen fertility, physical parameters,

certain routine sperm characteristics and testosterone in breeding Murrah buffalo (Bubalus bubalis) bulls. Vet World. 7:644–651. DOI:10.14202/vetworld.2014.644-651.

Singh K, Kumar A, Honparkhe M, Singh A. 2019. Quantification of Sexual Behaviour Traits in Presence of Teaser Male in Relation to Libido and Semen Quality in Buffalo Bulls. J Anim Res. 9:143–146. DOI: 10.30954/2277-940X.01.2019.19.

Singh S, Bhakat M, Mohanty TK, Kumar A, Gupta AK, Chakravarty AK, Singh P. 2015. Sexual behavior and its relationship with semen quality parameters in Sahiwal breeding bulls. Vet World. 8:745–749. DOI:10. 14202/vetworld.2015.745-749

Soren S, Singh SV, Singh P. 2016. Influence of season on seminal antioxidant enzymes in Karan Friesbulls under tropical climatic conditions. Turkish J Vet Anim Sci. 40:797–802. DOI:10.3906/vet-1603-76.

Souza LW de O, Andrade AFC, Celeghini ECC, Negrão JA, Arruda RP de. 2011. Correlation between sperm characteristics and testosterone in bovine seminal plasma by direct radioimmunoassay. Rev Bras Zootec. 40:2721–2724. DOI:10.1590/S1516-359820110012000 15.

Susilawati T. 2011. Spermatology. Malang (Indones): Universitas Brawijaya Press.

Syarifuddin NA, Toleng AL, Rahardja DP, Ismartoyo I, Yusuf M. 2017. Improving libido and sperm wuality of Bali bulls by supplementation of Moringa oleifera Leaves. Media Peternak. 40:88–93. DOI: 10.5398/medpet.2017. 40.2.88

Wang H-Q, Zhang W-D, Yuan B, Zhang J-B. 2021. Advances in the regulation of mammalian follicle-stimulating hormone secretion. Animals. 11:1134. DOI: 10.3390 /ani11041134

Widyaningrum Y, Luthfi M, Affandy L. 2015. Testosterone and Luteinizing hormone concentration of steer Ongolecrossbred in different housing models to accelerate puberty. In: Semin Nas Teknol Peternak dan Vet. Bogor (Indones): Pusat Penelitian dan Pengembangan Peternakan.

Zubair M, Ahmad M, Jamil H. 2015. Review on the screening of semen by hypo-osmotic swelling test. Andrologia. 47:744–750. DOI:10.1111/and.12335.

Refbacks

  • There are currently no refbacks.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.