Hide
Show all
Email this article 
Genetic Polymorphism of SCD1 Gene of Holstein-Friesian Cows in Indonesia
Abstract
Stearoyl-Coenzyme A desaturase 1 (SCD1) belongs to the fatty acid family of desaturases. In lactating ruminants, the SCD1 protein is highly expressed in the mammary gland and is relevant for the fatty acid composition of milk and dairy products. Polymorphism of SCD1 gene in Holstein-Friesian (HF) cows could be used as a basis of molecular selection of cattle in order to increase their productivity. The aim of this study was to investigate the polymorphism of SCD1 gene of Holstein-Friesian cows in Indonesia. A total of 162 blood samples of HF cows were collected from four different locations i.e. Bogor, Sukabumi, Tasikmalaya and Enrekang districts. Genotyping of SCD1 gene used PCR-RFLP method with NcoI restriction enzyme. The result showed that three genotypes (AA, AV and VV) and two alleles (A and V) have successfully found and polymorphic. A allele was dominant in all populations (0.63) and in Hardy Weinberg Equilibrium. The highest A allele was found in Sukabumi (0.78) and the lowest was in Bogor (0.55). Heterozigosity observed and expected reached 0.471 and 0.470, respectively. In conclusion, genetic polymorphism was found in all population with dominant of A allele. This finding can be used as a early genetic information of Holstein-Friesian cattle in Indonesia and to build breeding strategy for improving of productivity especially improving of healthy fat milk.Â
Keywords
Full Text:
PDFReferences
Allendorf FW and GL. 2017. Conservation and the Genetics of Population. In: Blackwell Publ. Malden, Massachusetts, USA.
Bhattacharya A, Banu J, Rahman M CJ and FG. 2006. Biological effects of conjugated linoleic acids in health and disease. J Nutr Biochem. 17:789–810.
Campbell, E. M. G., Gallagher, D. S., Davis, S. K., Taylor, J. F., and Smith SB. 2001. Mapping of the bovine stearoyl coenzyme A desaturase (SCD) gene to BTA26. J Anim Sci. 79:1954–1955.
Clark LA, Thomson JM, Moore SS, Oba M. 2010. The effect of Ala293Val single nucleotide polymorphism in the stearoyl-CoA desaturase gene on conjugated linoleic acid concentration in milk fat of dairy cows. Can J Anim Sci. 90:575–585.
Falconer DS, and T.F.C. Makay. 1996. Introduction to Quantitative Genetics, Ed 4. Longmans Green, Harlow, Essex, UK.
Jacobs AAA, Dijkstra J, Liesman JS, Vandehaar MJ, Lock AL, Vuuren AM Van, Hendriks WH, Baal J Van. 2013. Effects of short- and long-chain fatty acids on the expression of stearoyl-CoA desaturase and other lipogenic genes in bovine mammary epithelial cells. Animal. 7:9:1508–1516.
Kgwatalala PM, Mustafa AF, Zhao X. 2009. Influence of stearoyl-coenzyme A desaturase 1 genotype and stage of lactation on fatty acid composition of Canadian Jersey cows Stearoyl-CoA desaturase 1 genotype and stage of lactation influences milk fatty acid composition of Canadian Holstein cows. J Dairy Sci. 40:609–615.
Komisarek, J. and Dorynek Z. 2009. Effect of ABCG2, PPARGC1A, OLR1 and SCD1 gene polymorphism on estimated breeding values for functional and production traits in Polish Holstein-Friesian bulls. J Appl Genet. 50:125–132.
Kulig H, Zukowski K, Kowalewska-Luczak I, Lakomy P. 2016. SCD1 Polymorphism And Breeding Value For Milk Production Traits In Cows. Bulg J Agric Sci. 22:131–134.
Macciotta NPP, Mele M, Conte G, Serra A, Cassandro M, Zotto RD, Borlino AC, Pagnacco G, Secchiari P. 2008. Association Between a Polymorphism at the Stearoyl CoA Desaturase Locus and Milk Production Traits in Italian Holsteins. J Dairy Sci [Internet]. 91:3184–3189. Available from: http://dx.doi.org/10.3168/jds.2007-0947
Mashhadi MH. 2017. A Research on Association between SCD1 and OLR1 Genes and Milk Production Traits in Iranian Holstein Dairy Cattle. Iran J Appl Anim Sci. 7:243–248.
Mashhadi MH, Torshizi ME, Ahani S, Sobhanirad S, Bahari R. 2012. Polymorphism of SCD1 Gene in Iranian Holstein Dairy Cattle. In: Proc 15th AAAP Anim Sci Congr. Thailand; p. 838–842.
Maxin G, Rulquin H, Glasser F. 2011. Response of milk fat concentration and yield to nutrient supply in dairy cows. Animal. 5:8:1299–1310.
Mele M, Conte G, Castiglioni B, Chessa S, Macciotta NPP, Serra A, Buccioni A, Pagnacco G, Secchiari P. 2007. Stearoyl-Coenzyme A Desaturase Gene Polymorphism and Milk Fatty Acid Composition in Italian Holsteins Stearoyl-Coenzyme A Desaturase Gene Polymorphism and Milk Fatty. J Dairy Sci. 90:4458–4465.
Milannesi E, Nicoloso L, Crepald P. 2008. SCD gene polymorphisms in Italian cattle breeds. J Anim Breed Genet. 125:63–67.
Moioli, B., Contarini, G., Avalli, A., Cavillo, G., Orr, L., De Matteis, G., Masoero, G. and Napoletano F. 2007. Effect of stearoyl coenzyme A desaturase polymorphism on fatty acid composition of milk. J Dairy Sci. 90:3553–3558.
Nei M and SK. 2000. Molecular Evolution and Phylogenetics. New York: Oxford University Press.
Ntambi JM and MM. 2004. Regulation of Stearoyl-CoA Desaturases and Role in Metabolism. ProgLipid Res. 43:91–104.
Reynolds CM and Roche HM. 2010. Conjugated linoleic acid and inflammatory cell signaling. Prostaglandins, Leukot Essent Fat Acids. 82:199–204.
Schennink, A., Heck, J.M., Bovenhuis, H., Visker, M.H., Van Valenberg, H.J. and Van Arendonk JA. 2008. Milk fatty acid unsaturation: Genetic parameters and effects of stearoyl-CoA desaturase (SCD1) and acyl CoA: diacylglycerol acyltransferase 1 (DGAT1). J Dairy Sci. 91:2135–2143.
Soltani-Ghombavani M, Ansari-Mahyari S, Rostami M, Ghanbari-Baghenoei S, Edriss MA. 2016. Effect of polymorphisms in the ABCG2 , LEPR and SCD1 genes on milk production traits in Holstein cows. S Afr J Anim Sci. 46:196–203.
Taniguchi, M., T. Utsugi, K. Oyama HM, M. Kobayashi, Y. Tanabe AO and S, Tsuji. 2004. Mamm Genome. Genotype stearoyl-CoA desaturase is Assoc with Fat acid Compos Japanese Black Cattle. 14:142–148.
Refbacks
- There are currently no refbacks.
This work is licensed under a Creative Commons Attribution 4.0 International License.
