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Aflatoxin M1 in fresh dairy milk from small individual farms in Indonesia
Abstract
This present study was aimed to investigate the presence of aflatoxin M1 (AFM1) residue in fresh dairy milk collected from small dairyl farms. A total of 104 samples of fresh cow's milk were collected in Pengalengan-Bandung and Sukabumi (West Java province), and Tanggamus (Lampung province) in April and September 2012. All samples were analyzed by a high performance liquid chromatography and detected with fluorescence detector after extraction with organic solvents. Contamination of AFM1 was found on 1.96% (1/51) from the samples collected in April 2012 at concentration of 1.20 ng/L and 39.63% (21/53) from the samples collected in September 2012 at concentration of 1.20 ng/L 1.0 – 34.1 ng/L. Those positive samples were obtained from Pangalengan and Sukabumi, but none for those samples collected from Tanggamus both on collection time April nor September 2012. In those positive samples for AFM1, there is no sample contained AFM1 above the maximum level (ML) regulated in Indonesia (500 ng/L or 0.5 µg/L). Low contamination levels of AFB1 in the range of 0.38 to 6.64 µg/kg found in supplemental feed samples from the same sampling time and locations. The findings of AFM1 contamination in raw fresh milk from this study caused no harm to the consumers. However, regular monitoring on the presence of AFM1 in dairy milk and aflatoxin B1 (AFB1) in dairy cattle feed is necessary to ensure the protection of human health.
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Agus A, Khuluq I, Sumantri I, Noviandi CT, Nuryono. 2010. Aflatoxin M1 excretion in the milk of tropical dairy cow fed contaminated aflatoxin B1 in the diet. The 5th International Seminar on Tropical Animal Production Community Empowerment and Tropical Animal Industry. Yogyakarta (Indonesia). p. 282-285.
Agus A, Sumantri I, Murti TW, Boehm J. 2013. Survey on the occurence of aflatoxin b1 contamination in dairy ration and its carry over into the milk in Yogyakarta and Central Java Provinces of Indonesia. Paper for Presented in ISM-Mycored International Conference Europe 2013.
Anukul N, Vangnai K, Mahakarnchanakul. 2013. Significance of regulation limits in mycotoxin contamination in Asia and risk management programs at the national level. J Food Drug Analysis. 21:227-241.
Asi MR, Iqbal SZ, Arino A, Hussain A. 2012. Effect of seasonal variations and lactation times on aflatoxin M1 contaminationin milk of different species from Punjab, Pakistan. Food Control. 25:34-38.
[BSN] Badan Standarisasi Nasional. 2009. SNI 7385:2009. Batas maksimum mikotoksin dalam pangan. Jakarta (Indones): Badan Standarisasi Nasional.
[BPOM] Badan Pengawas Obat dan Makanan Republik Indonesia. 2009. Peraturan Kepala Badan Pengawas Obat dan Makanan Republik Indonesia. Nomor HK.00.06.1.52.4011 tentang Penetapan Batas Maksimum Cemaran Mikroba dan Kimia dalam Makanan. Jakarta (Indones): Badan Pengawas Obat dan Makanan Republik Indonesia.
[BSN] Badan Standarisasi Nasional. 2009. SNI. 3148.1:2009. Pakan konsentrat – Bagian I: Sapi Perah. Jakarta (Indones): Badan Standarisasi Nasional.
Bilandžić N, Varenina I, Solomun K, Božica, Božić L, ÄurÄ‘ica, Varga I, Želježić B, Cvetnić L, Benić M, Tanković S, Cvetnić Ž. 2017. Occurrence of aflatoxin M1 in raw cow, goat and sheep milk during spring and autumn in Croatia during 2016. Toxin Reviews. 36:1-7.
Codex Allimentarius. 2015. General standard for contaminants and toxins in food and feed (Codex STAN 193-1995). [accessed August 28th 2018]. www.fao.org/input/download/.../17/CXS_193e_2015.pdf
Creppy EE. 2002. Update of survey, regulation and toxic effects of mycotoxins in Europe. Toxicol Lett. 127:19-28.
van Egmond HP. 1989. Mycotoxins in dairy products. Aflatoxin M1: Occurrence, Toxicity, Regulation. London (UK): Elsevier Applied Science. p. 11–55.
Fernandes AM, Corrêa B, Rosim RE, Kobashigawa E, Oliveira CAF. 2012. Distribution and stability of aflatoxin M1 during processing and storage of Minas Frescal cheese. Food Control. 24:104-108.
Fillaeli A. 2007. Cemaran alami aflatoksin B1 dalam pakan konsentrat dan aflatoksin M1 dalam susu sapi perah (Thesis). [Yogyakarta (Indones)]: Gadjah Mada University.
[FAO] Food and Agriculture Organization. 2004. Worldwide regulations for mycotoxins in food and feed in 2003. Feed and Nutrition Paper 81. Rome (Italy): Food and Agriculture Organization of the United Nations.
Goncalves L, Dalla Rosa A, Gonzales SL, Feltes MC, Baliale-Furlong E, Dors GC. 2017. Incidence of aflatoxin M1 in fresh milk from small farms. Food Sci Technol Campinas. 37:11-15.
Kang’Ethe EK, Lang’AKA. 2009. Aflatoxin B1 and M1 contamination of animal feeds and milk from urban centers in Kenya. Afr Health Sci. 9:218-226.
Kılıç Altun S, Gürbüz S, Ayağ E. 2017. Aflatoxin M1 in human breast milk in southeastern Turkey. Mycotoxin Res. 33:103-107.
Mahmoudi R, Norian R. 2015. Aflatoxin B1 and M1 contamination in cow feeds and milk from Iran. Food Agric Immunol. 26:131-137.
Nadira AF, Rosita J, Norhaizan ME, Redzwan SM. 2017. Screening of aflatoxin M1 occurrence in selected milk and dairy products in Terengganu, Malaysia. Food Control. 73:209-214.
Nuryono N, Agus A,Wedhastri S, Maryudani YB, Setyabudi FMCS, Bohm J, Razzazi-Fazeli E. 2009. A limited survey of aflatoxin M1 in milk from Indonesia by ELISA. Food Control. 20:721-724
Nurhayati D. 2014. Deteksi aflatoksin M1 pada susu pasteurisasi (Thesis). [Bogor (Indones)]: of Bogor Agricultural University.
Ostry V, Malir F, Toman J, Grosse Y. 2017. Mycotoxins as humans carcinogens-The IARC Monograph classification. Mycotoxin Res. 33:63-75.
Prandini A, Tansini G, Sigolo S, Filippi L, Laporta M, Piva G. 2009. On the occurrence of aflatoxin M1 in milk and dairy products. Food Chem Toxicol. 47:984-91.
Picinin LCA, Cerqueira MMOP, Vargas EA, Lana ÂMOQ, ToaldoI M, Bordignon-Luiz MT. 2013. Influence of climate conditions on aflatoxin M1 contamination in raw milk from Minas Gerais State, Brazil. Food Control. 31:419-424.
Ruangwises N, Ruangwises S, 2010. Aflatoxin M1 contamination in raw milk within the central region of Thailand. Bull Environ Contam Toxicol. 85:195-198.
Șanlı T, Deveci O, Sezgİn E. 2012. Effects of pasteurization and storage on stability of aflatoxin M1 in yogurt. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 18:987-990.
Shrivastava A, Gupta VB. 2011. Methods for the determination of limit of detection and limit of quantitation of the analytical methods. Chronicles Young Scientists. 2:21-25.
Shuib NS, Makahleh A, Salhimi SM, Saad B. 2017. Natural occurrence of aflatoxin M1 in fresh cow milk an human milk. Food Control. 73:966-970.
Sumantri I, Murti TW, Van der Poel AFB, Boehm J, Agus A. 2012. Carry-over of aflatoxin B1-feed into aflatoxin M1-milk in dairy cows treated with natural sources of aflatoxin and bentonite. J Indones Trop Anim Agric. 37:271-277.
Widiastuti R, Maryam R, Bahri S. 2006. Aflatoxin M1 (AFM1) in fresh dairy milk in Pangalengan and Bogor Districts, West Java. Mathius IW, Sendow I, Nurhayati, Murdiati TB, Thalib A, Beriajaya, Suparyanto A, Prasetyo LH, Darmono, Wina E, editors. Proceeding of Seminar Nasional Teknologi Peternakan dan Veteriner. Bogor (Indones): Pusat Penelitian dan Pengembangan Peternakan. p. 239-243.
Widiastuti R, Indraningsih, Firmansyah R. 2009. Analysis of aflatoxin in corn which purified with SPE silica and detected with HPLC. Sani Y, Martindah E, Nurhayati, Puastuti W, Sartika T, Parede L, Anggraeni A, Natalia L, editors. Proceeding of Seminar Nasional Teknologi Peternakan dan Veteriner. Bogor (Indones): Pusat Penelitian dan Pengembangan Peternakan. p. 705-710.
Wijaya HR, Widjajanti NI, Silitonga RF. 2018. Detection of aflatoxin M1 in powdered milk and sweetened condensed milk products in several cities in Java with HPLC-fluorescence method. International Symposium on Food and Agro-biodiversity (ISFA) 2017. IOP Conference. Series: Earth and Environmental Science. 102:012028.
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